Type 2 Nep1-Like Proteins from the Biocontrol Oomycete Pythium oligandrum Suppress Phytophthora capsici Infection in Solanaceous Plants.
Type 2 Nep1-Like Proteins from the Biocontrol Oomycete Pythium oligandrum Suppress Phytophthora capsici Infection in Solanaceous Plants.
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来自生物防治卵菌寡雄腐霉的 2 型 Nep1 样蛋白可抑制茄科植物的辣椒疫霉感染
DOI：
10.3390/jof7070496
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发表时间：
2021-06-22
期刊：
影响因子：
--
通讯作者：
Dou D
中科院分区：
文献类型：
--
作者：
Yang K;Dong X;Li J;Wang Y;Cheng Y;Zhai Y;Li X;Wei L;Jing M;Dou D
寡雄腐霉是一种非致病性卵菌，能通过直接的真菌寄生活性和增强植物的免疫反应来控制植物病害。已经发现几种寡雄假单胞菌激发子作为微生物相关分子模式（MAMPs）激活植物免疫。坏死和乙烯诱导肽1（Nep 1）样蛋白（NLP）是一类广泛存在于真核和原核植物病原体中的MAMPs。然而，对寡雄腐霉及其姊妹种Pythiumperiplocum中的分布和功能知之甚少。在此，我们鉴定了来自寡雄拟单胞菌（PyolNLP）和围盖拟单胞菌（PypeNLP）的总共25个NLP。同时，我们发现PyolNLPs/PypeNLPs基因聚集在两个染色体片段上，我们的分析表明，它们通过复制扩展，并且具有与致病性卵菌完全不同的共同起源。9种PyolNLP/PypeNLP通过农杆菌渗入诱导本氏烟草坏死。测试了8种部分纯化的PyolNLP/PypeNLP的潜在生物防治活性。PyolNLP 5和PyolNLP 7在N.通过直接蛋白质浸润的本塞姆氏菌。在足够的浓度下，它们都能显著降低辣椒疫霉在茄科植物中的病害严重程度，并抑制辣椒疫霉在茄科植物中的生长。烟草（烟草）、番茄（Solanum lycopersicum）和辣椒（Capsicum annuum）。我们的测定表明PyolNLP 5和PyolNLP 7的疫霉抑制作用与活性氧（ROS）积累无关。相反，它们诱导抗微生物植物防御素基因的表达，并且诱导依赖于它们保守的nlp 24样肽模式。本工作证明了两种寡雄拟青霉NLP对茄科植物的生物防治作用，揭示了利用NLP开发用于卵菌病原体防治的生物活性配方而不会对植物造成ROS损伤的新方法。
As a non-pathogenic oomycete, the biocontrol agent Pythium oligandrum is able to control plant diseases through direct mycoparasite activity and boosting plant immune responses. Several P. oligandrum elicitors have been found to activate plant immunity as microbe-associated molecular patterns (MAMPs). Necrosis- and ethylene-inducing peptide 1 (Nep1)-like proteins (NLPs) are a group of MAMPs widely distributed in eukaryotic and prokaryotic plant pathogens. However, little is known about their distribution and functions in P. oligandrum and its sister species Pythium periplocum. Here, we identified a total of 25 NLPs from P. oligandrum (PyolNLPs) and P. periplocum (PypeNLPs). Meanwhile, we found that PyolNLPs/PypeNLPs genes cluster in two chromosomal segments, and our analysis suggests that they expand by duplication and share a common origin totally different from that of pathogenic oomycetes. Nine PyolNLPs/PypeNLPs induced necrosis in Nicotiana benthamiana by agroinfiltration. Eight partially purified PyolNLPs/PypeNLPs were tested for their potential biocontrol activity. PyolNLP5 and PyolNLP7 showed necrosis-inducing activity in N. benthamiana via direct protein infiltration. At sufficient concentrations, they both significantly reduced disease severity and suppressed the in planta growth of Phytophthora capsici in solanaceous plants including N. benthamiana (tobacco), Solanum lycopersicum (tomato) and Capsicum annuum (pepper). Our assays suggest that the Phytophthora suppression effect of PyolNLP5 and PyolNLP7 is irrelevant to reactive oxygen species (ROS) accumulation. Instead, they induce the expression of antimicrobial plant defensin genes, and the induction depends on their conserved nlp24-like peptide pattern. This work demonstrates the biocontrol role of two P. oligandrum NLPs for solanaceous plants, which uncovers a novel approach of utilizing NLPs to develop bioactive formulae for oomycete pathogen control with no ROS-caused injury to plants.
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影响因子：
3.5
作者：
Kanneganti, Thirumala-Devi;Huitema, Edgar;Kamoun, Sophien
通讯作者：
Kamoun, Sophien
影响因子：
10.7
作者：
Jiang, RHY;Tyler, BM;Govers, F
通讯作者：
Govers, F
影响因子：
5.5
作者：
Ali-Shtayeh, MS;Saleh, ASF
通讯作者：
Saleh, ASF
影响因子：
5.8
作者：
Gerbore, J.;Benhamou, N.;Rey, P.
通讯作者：
Rey, P.
DOI：
10.1128/genomea.00346-17
发表时间：
2017-05-25
期刊：
Genome announcements
影响因子：
--
作者：
Kushwaha SK;Vetukuri RR;Grenville-Briggs LJ
通讯作者：
Grenville-Briggs LJ