Transplantation of bone marrow stromal cell-derived Schwann cells promotes axonal regeneration and functional recovery after complete transection of adult rat spinal cord
Transplantation of bone marrow stromal cell-derived Schwann cells promotes axonal regeneration and functional recovery after complete transection of adult rat spinal cord
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DOI：
10.1093/jnen/64.1.37
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发表时间：
2005-01-01
影响因子：
3.2
通讯作者：
Yamazaki, M
中科院分区：
文献类型：
--
作者：
Kamada, T;Koda, M;Yamazaki, M
本研究的目的是评估移植来自骨髓基质细胞（BMSC-SCs）的雪旺细胞是否促进成年大鼠完全横断脊髓的轴突再生和功能恢复。体外诱导骨髓基质细胞向雪旺细胞分化。在T7水平完全切除大鼠脊髓的4 mm节段。将填充有Matrigel（MG）和BMSC-SC的混合物（BMSC-SC组）或仅填充有Matrigel（MG组）的超滤膜管移植到差距中。在BMSC-SC组，神经丝和酪氨酸羟化酶免疫反应阳性神经纤维的数量显着高于MG组，虽然5-羟色胺或降钙素基因相关肽免疫反应阳性纤维在两组中很少检测到。在BMSC-SC组中，后肢功能明显恢复，但移植后6周再次切断移植物后，后肢功能恢复消失。这些结果表明，骨髓间充质干细胞移植促进损伤脊髓的轴突再生，导致大鼠后肢功能的恢复。骨髓间充质干细胞移植是治疗脊髓损伤的一种有效方法。
The aim of this study was to evaluate whether transplantation of Schwann cells derived from bone marrow stromal cells (BMSC-SCs) promotes axonal regeneration and functional recovery in completely transected spinal cord in adult rats. Bone marrow stromal cells (BMSCs) were induced to differentiate into Schwann cells in vitro. A 4-mm segment of rat spinal cord was removed completely at the T7 level. An ultra-filtration membrane tube, filled with a mixture of Matrigel (MG) and BMSC-SCs (BMSC-SC group) or Matrigel alone (MG group), was grafted into the gap. In the BMSC-SC group, the number of neurofilament- and tyrosine hydroxylase-immunoreactive nerve fibers was significantly higher compared to the MG group, although 5-hydroxytryptamine- or calcitonin gene-related peptide-immunoreactive fibers were rarely detectable in both groups. In the BMSC-SC group, significant recovery of the hindlimb function was recognized, which was abolished by retransection of the graft 6 weeks after transplantation. These results demonstrate that transplantation of BMSC-SCs promotes axonal regeneration of lesioned spinal cord, resulting in recovery of hindlimb function in rats. Transplantation of BMSC-SCs is a potentially useful treatment for spinal cord injury.