Biochemical and Physicochemical Background of Mammalian Androgen Activity in Winter Wheat Exposed to Low Temperature
Biochemical and Physicochemical Background of Mammalian Androgen Activity in Winter Wheat Exposed to Low Temperature
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DOI：
10.1007/s00344-017-9719-1
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发表时间：
2018-03-01
影响因子：
4.8
通讯作者：
Skoczowski, Andrzej
中科院分区：
文献类型：
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作者：
Janeczko, Anna;Biesaga-Koscielniak, Jolanta;Skoczowski, Andrzej
对哺乳动物激素雄烯二酮（AN）在植物中的生理作用的了解很少，其在细胞水平上的作用机制几乎是未知的。本研究旨在探讨低温条件下冬小麦a酶活性的理化生化背景。寒冷时期在这个物种的生命周期中是重要的，因为它们诱导抗冻性和进一步的生殖发育。小麦幼苗（对照和补氮素对照）在低温环境下驯化2周，然后暴露于霜冻环境（-12℃）。AN的补充减少了30%的霜冻伤害。此外，氨氮还能促进小麦的生殖发育。an诱导的氧化还原稳态变化似乎对低温驯化和生殖诱导过程很重要。AN影响小麦的激素平衡，刺激其他赤霉素和细胞分裂素的积累。例如，在植物地上部分，AN在12 d的冷处理中使GA(3)含量增加了约30%，而顺式玉米素含量增加了65%。AN被植物膜吸收（Langmuir浴研究）。氨氮的膜吸收增加了脂质分子之间的距离，这可能是氨氮诱导的抗冻性增强的重要步骤。与脂质膜的相互作用与一些已知的植物开花调节因子的相互作用相似，因此它也可能是小麦发育加速的基础。小麦叶片中天然存在雄烯二酮（5-21 pg g(-1) FW）。
Understanding of the physiological role of mammalian hormone-androstenedione (AN)-in plants is scant and the mechanisms of its action at a cellular level are practically unknown. The aim of this study was to investigate the physicochemical and biochemical background of AN activity in winter wheat exposed to low temperature. Cold periods are important in the lifecycle of this species as they induce frost resistance and further generative development. Wheat seedlings (control and AN-supplemented) were acclimated 2 weeks in cold and then exposed to frost (-12 A degrees C). AN supplementation reduced frost damages by 30%. Moreover, AN also accelerated generative development of wheat. The AN-induced changes in redox homeostasis seemed to be important for processes of acclimation to low temperature and generative induction. AN influenced hormonal balance in wheat and stimulated accumulation among other gibberellins and cytokinins. For example, in aerial part of plants, the content of GA(3) was increased by AN in 12 days of cold by about 30%, whereas the content of cis-zeatin was increased by 65%. AN was absorbed into plant membranes (Langmuir bath studies). The membrane absorption of AN increased the distance between lipid molecules and this may be an important step in the AN-induced enhancement of frost resistance. AN interaction with lipid membranes showed similarity to the interactions of some known regulators stimulating flowering in plants, and thus it may also underlie the acceleration of wheat development. Androstenedione was naturally present in wheat leaves (5-21 pg g(-1) FW).