A NOVEL PROTEIN PRODUCED BY THE VITELLOGENIC FAT-BODY AND ACCUMULATED IN MOSQUITO OOCYTES
A NOVEL PROTEIN PRODUCED BY THE VITELLOGENIC FAT-BODY AND ACCUMULATED IN MOSQUITO OOCYTES
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DOI：
10.1007/bf02029559
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发表时间：
1990-01-01
期刊：
影响因子：
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通讯作者：
RAIKHEL, AS
中科院分区：
文献类型：
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作者：
HAYS, AR;RAIKHEL, AS
卵黄发生蚊的脂肪体被发现合成和分泌除卵黄蛋白原之外的另一种蛋白质，其积累在发育中的卵母细胞中。在组织中，该蛋白在还原或非还原条件下的SDS-PAGE上具有Mr = 53000。该蛋白质被糖基化，如[3 H]甘露糖掺入和衣霉素实验所示。使用卵巢53-kDa肽产生多克隆抗体。免疫印迹分析证明了来自脂肪体和卵巢的53 kDa肽的免疫同一性。此外，53-kDa蛋白（53 KP）仅由卵黄发生脂肪体合成和分泌。放射免疫分析表明，53 KP在卵黄发生开始后4 h即由雌体脂肪体产生，24 h左右达到高峰。然后，合成在36小时降至低水平，并在48小时降至背景水平。对睾丸激素前雌性脂肪体进行的体外实验表明，生理剂量的20-羟蜕皮酮（10-6 M）可以刺激53 KP的合成和分泌。卵巢的免疫细胞化学研究表明，53 KP存在于卵泡细胞之间的通道，在卵周间隙和发育中的卵母细胞的卵黄颗粒。这表明53 KP在卵母细胞中的积累途径与卵黄蛋白原类似。
The fat body of vitellogenic mosquitoes was found to synthesize and secrete another protein in addition to vitellogenin, that accumulated in developing oocytes. In the tissues, this protein has Mr = 53000 on SDS-PAGE under reducing or non-reducing conditions. This protein is glycosylated as shown by [3H]mannose incorporation and experiments with tunicamycin. Polyclonal antibodies were produced using the ovarian 53-kDa peptide. Immunoblot analysis demonstrated the immunological identity of 53 kDa peptides from the fat body and the ovary. Furthermore, the 53-kDa protein (53KP) is synthesized and secreted exclusively by the vitellogenic fat body. Radioimmunoassay showed that 53KP is produced by the female fat body as early as 4 h and reaches its peak near 24 h after the initiation of vitellogenesis. Synthesis then drops to low levels by 36 h and declines to background levels by 48 h. In vitro experiments conducted on fat bodies of previtellogenic females demonstrated that the synthesis and secretion of 53KP can be stimulated by a physiological dose of 20-hydoxyecdysone (10-6 M). Immunocytochemical studies of the ovary demonstrate that 53KP is present in channels between follicle cells, in the perioocytic space and in yolk granules of the developing oocytes. This suggests that 53KP is accumulated in the oocytes by a pathway similar to that of vitellogenin.