Simultaneous determination of heparan sulfate, chondroitin/dermatan sulfates, and hyaluronan glycosaminoglycan disaccharides by high-performance liquid chromatography using a reverse-phase column with adamantyl groups
Simultaneous determination of heparan sulfate, chondroitin/dermatan sulfates, and hyaluronan glycosaminoglycan disaccharides by high-performance liquid chromatography using a reverse-phase column with adamantyl groups
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使用金刚烷基反相柱通过高效液相色谱同时测定硫酸乙酰肝素、硫酸软骨素/硫酸皮肤素和透明质酸糖胺聚糖二糖
DOI：
10.1016/j.chroma.2022.463748
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发表时间：
2023
影响因子：
4.1
通讯作者：
Furukawa Jun-ichi
中科院分区：
文献类型：
--
作者：
Hanamatsu Hisatoshi;Makino Satoshi;Ohara Masatsugu;Suda Goki;Yokota Ikuko;Nishihara Shoko;Sakamoto Naoya;Furukawa Jun-ichi
糖胺聚糖（Glycosaminoglycans，GAGs）是蛋白聚糖的主要成分之一，在信号转导、细胞粘附、生长和分化等生理过程中发挥着重要作用。由于具有大量硫酸酯或羧基的杂多糖的巨大结构多样性，GAG的表征具有挑战性。在本研究中，我们研究了分析2-氨基苯甲酰胺（2-AB）标记的GAG二糖的高效液相色谱法（HPLC）使用反相（RP）柱与金刚烷基团。在分析条件下，在一次分析中依次分离了17种2-AB标记的GAG二糖，这些二糖来源于硫酸乙酰肝素、硫酸软骨素/皮肤素和透明质酸。分析时间快，保留时间重现性好。此外，具有金刚烷基基团的RP-HPLC柱允许定量各种生物样品（例如血清、培养细胞和培养基）中的GAG。
Glycosaminoglycans (GAGs), which are one of the major components of proteoglycans, play a pivotal role in physiological processes such as signal transduction, cell adhesion, growth, and differentiation. Characterization of GAGs is challenging due to the tremendous structural diversity of heteropolysaccharides with numerous sulfate or carboxyl groups. In this present study, we examined the analysis of 2-aminobenzamide (2-AB) labeled GAG disaccharides by high-performance liquid chromatography (HPLC) using a reverse-phase (RP)-column with adamantyl groups. Under the analytical conditions, 17 types of 2-AB labeled GAG disaccharides derived from heparan sulfate, chondroitin/dermatan sulfates, and hyaluronan were sequentially separated in a single analysis. The analysis time was fast with high retention time reproducibility. Moreover, the RP-HPLC column with adamantyl groups allowed the quantification of GAGs in various biological samples, such as serum, cultured cells, and culture medium.