Inhalation of low-level formaldehyde increases the Bcl-2/Bax expression ratio in the hippocampus of immunologically sensitized mice
Inhalation of low-level formaldehyde increases the Bcl-2/Bax expression ratio in the hippocampus of immunologically sensitized mice
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DOI：
10.1159/000094829
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发表时间：
2006-01-01
影响因子：
2.4
通讯作者：
Fujimaki, Hidekazu
中科院分区：
文献类型：
--
作者：
Tsukahara, Shinji;Yamamoto, Shoji;Fujimaki, Hidekazu
目的：我们研究小组最近的一项研究表明，反复暴露于低水平甲醛（FA）会增加免疫小鼠海马中神经生长因子的产生，涉及神经元的存活和维持。在本研究中，我们研究了FA对调节细胞存活和死亡的凋亡机制的影响，以及对小鼠海马中与海马功能相关的N-甲基-D-天冬氨酸（NMDA）受体的影响。研究方法：Western印迹分析Bcl-2，Bax和NMDA受体亚型2A和2B的海马取自C3 H小鼠暴露于0或400 ppb的FA与或不与卵清蛋白（OVA）免疫。还对这些小鼠进行了活性caspase-3的免疫组织化学分析。结果如下：Bcl-2 Bax的表达水平的比例显着增加与400 ppb FA暴露在OVA免疫的小鼠，但没有在没有OVA免疫的小鼠，虽然在每个蛋白质水平的差异不显着组之间。海马内可见caspase-3免疫反应阳性细胞。然而，这一数字只是少数，并没有显着影响FA暴露和OVA免疫。NMDA受体2A型和2B型表达水平的FA暴露的小鼠持续在与对照小鼠有或没有OVA免疫的水平相当。结论：这些结果表明，Bcl-2/Bax表达比例的变化，发生与低水平FA暴露和免疫，并可能遵循增强神经生长因子的生产，发挥了保护作用，对细胞凋亡的细胞死亡。版权所有（c）2006 S. Karger AG，巴塞尔。
Objective: A recent study from our research group showed that repeated exposure to low-level formaldehyde (FA) increases the production of nerve growth factor, involving the survival and maintenance of neurons, in the hippocampus of immunized mice. In the present study, we examined the effects of FA on apoptotic mechanisms regulating survival and death of cells and on N-methyl-D-aspartate (NMDA) receptors related to hippocampal functions in the mouse hippocampus. Methods: Western blot analyses were performed for Bcl-2, Bax and NMDA receptor subtypes 2A and 2B of the hippocampus taken from C3H mice exposed to 0 or 400 ppb of FA with or without ovalbumin (OVA) immunization. Immunohistochemical analysis for active caspase-3 was also carried out for these mice. Results: The ratio of Bcl-2 to Bax expression levels significantly increased with 400-ppb FA exposure in OVA-immunized mice but not in mice without OVA immunization, although differences in each protein level were not significant among groups. Active caspase-3-immunoreactive cells were found in the hippocampus. However, the number was only a few and not significantly affected by FA exposure and OVA immunization. NMDA receptor type 2A and 2B expression levels of FA-exposed mice were sustained at comparative levels with those for the control mice with or without OVA immunization. Conclusions: These results indicate that changes in the Bcl-2/Bax expression ratio, which occurs with low-level FA exposure and immunization and may follow enhancement of nerve growth factor production, exerts a protective effect against cell death by apoptosis. Copyright (c) 2006 S. Karger AG, Basel.