COMPARATIVE-STUDY OF THE REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF BLACK TEA LIQUORS WITH SPECIAL REFERENCE TO THE THEARUBIGINS
COMPARATIVE-STUDY OF THE REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF BLACK TEA LIQUORS WITH SPECIAL REFERENCE TO THE THEARUBIGINS
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DOI：
10.1016/s0021-9673(01)88752-5
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发表时间：
1991-03-29
期刊：
影响因子：
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通讯作者：
MCDOWELL, I
中科院分区：
文献类型：
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作者：
BAILEY, RG;NURSTEN, HE;MCDOWELL, I
介绍了用Hypersil ODS柱、Hypersil辛基宽孔色谱柱和HAMILTON PRP-1色谱柱分析红茶酚类色素的新方法，以及由此产生的几个新结果。使用Hypersil ODS色谱柱和柠檬酸盐缓冲液获得了非常好的分辨率，并且第一次在一个色谱图中观察到了八种茶黄素。未溶解的棕色酚醛颜料(茶红素)在Hypersil宽孔和Hamilton PRP-1柱上呈凸起的宽带，而Hamilton PRP-1柱显示花青素是白酒颜色的重要贡献者。根据色层行为将颜料分为三组：第一组，接近色谱柱空隙体积的颜料；第二组，可分辨颜料；以及第三组，未溶解色素。根据它们的光电二极管阵列UV-Vis光谱，第二组色素被分为四个亚组，如下：亚组II.1，茶黄素；亚组II.2，茶黄酸；亚组II3，第一类溶解色素；以及亚组II4，第二类分辨色素。亚组II.3和亚组II.4中的色素被命名为可溶红素，而组III中的色素被命名为未溶红素。
New high-performance liquid chromatographic methods for the analysis of black tea phenolic pigments, using Hypersil ODS, Hypersil octyl wide-pore, and Hamilton PRP-1 columns, and several new results arising from them are presented. Very good resolution of a wide range of phenolic tea pigments, was obtained using a Hypersil ODS column with a citrate buffer, and, for the first time, eight theaflavins were observed in a single chromatogram. Unresolved brown phenolic pigments (thearubigins), ran as a convex broad band on the Hypersil wide-pore and Hamilton PRP-1 columns, and the Hamilton PRP-1 column showed an anthocyanidin to be a significant contributor to liquor colour. The pigments were classified into three groups by chromatographic behaviour: group I, pigments running close to the void volume of the columns; group II, resolved pigments; and group III, unresolved pigments. Group II pigments were divided into four Sub-Groups by their photodiode-array UV-VIS spectra, as follows: subgroup II.1, theaflavins; sub-group II.2, theaflavic acids; sub-group II.3, type I resolved pigments; and sub-group II.4, type II resolved pigments. Pigments in sub-groups II.3 and II.4 were designated as resolved thearubigins, and those in group III as unresolved thearubigins.