Quantification of bisphenol A and its selected analogs in serum using pre-column derivatization with high-performance liquid chromatography and tandem mass spectrometry
Quantification of bisphenol A and its selected analogs in serum using pre-column derivatization with high-performance liquid chromatography and tandem mass spectrometry
复制标题
使用柱前衍生化、高效液相色谱和串联质谱法对血清中的双酚 A 及其选定类似物进行定量
DOI：
10.1002/jssc.201800847
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发表时间：
2019-03
影响因子：
3.1
通讯作者：
Chen Jiping
中科院分区：
文献类型：
--
作者：
Tan Dongqin;Jin Jing;Wang Longxing;He Xiaolin;Guo Cuicui;Dhanjai;Lu Xianbo;Chen Jiping
由于双酚A的使用受到管制，作为双酚A替代品的几种类似物因其对健康的不利影响而引起了人们的极大关注。为了调查它们在人体中的发生并确定可能的污染源，有必要开发一种灵敏的总双酚检测方法。因此，提出了一种基于反相和液-液萃取，然后分子印迹聚合物固相萃取和柱前衍生化与高效液相色谱和串联质谱的方法。该方法具有良好的上级选择性和灵敏度。可以在很大程度上消除基体效应。方法检出限为0.05 ~ 0.19 ng/mL。通过在三个水平（0.8、8和20 ng/mL）下加标牛血清，获得了令人满意的回收率（71相似于119%）。该方法已成功地应用于儿童血清中总双酚的测定。检测到双酚A、双酚F、双酚S、双酚B和双酚F的浓度分别低于方法检测限至1.65、0.45、0.79、2.04和0.17 ng/mL。这些结果表明，双酚A仍然是主要的污染物中研究的双酚在儿童中，而双酚A类似物的威胁也应加以监测。
Due to regulation of the use of bisphenol A, several analogs serving as bisphenol A replacements have drawn substantial attention for their adverse health effects. To investigate their occurrence in humans and identify possible pollution sources, it is necessary to develop a sensitive method for total bisphenols detection. Thus, a method based on enzymolysis and liquid-liquid extraction followed by molecularly imprinted polymer solid-phase extraction and pre-column derivatization with high-performance liquid chromatography and tandem mass spectrometry was proposed. The developed method exhibited superior selectivity and sensitivity. The matrix effect can be eliminated to a great extent. The method detection limits for eight bisphenols were 0.05 similar to 0.19 ng/mL. Satisfactory recoveries (71 similar to 119%) were obtained by spiking bovine serum at three levels (0.8, 8 and, 20 ng/mL). The method was successfully applied to determine total bisphenols in the serum samples of children. Bisphenol A, bisphenol F, bisphenol S, bisphenol B and bisphenol F were detected with concentrations from below the method detection limit to 1.65, 0.45, 0.79, 2.04 and 0.17 ng/mL, respectively. These results indicate that bisphenol A remains the major pollutant among the studied bisphenols in children, whereas threats from bisphenol A analogs should also be monitored.