COLOCALIZATION OF N-ACETYL-ASPARTYL-GLUTAMATE IN CENTRAL CHOLINERGIC, NORADRENERGIC, AND SEROTONERGIC NEURONS
COLOCALIZATION OF N-ACETYL-ASPARTYL-GLUTAMATE IN CENTRAL CHOLINERGIC, NORADRENERGIC, AND SEROTONERGIC NEURONS
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DOI：
10.1002/syn.890010509
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发表时间：
1987-01-01
期刊：
影响因子：
2.3
通讯作者：
COYLE, JT
中科院分区：
文献类型：
--
作者：
FORLONI, G;GRZANNA, R;COYLE, JT
同时显示两种不同抗原的免疫组织化学技术已被用于研究CNS内胆碱能、去甲肾上腺素能-肾上腺素能和多巴胺能神经元中酸性二肽N-乙酰基-γ-谷氨酸（NAAG）的存在。用纯化的NAAG抗血清处理脑切片，然后用胆碱乙酰转移酶（ChAT）单克隆抗体（胆碱能神经元的标记物）或多巴胺-β-羟化酶（DBH）抗血清（去甲肾上腺素能-肾上腺素能神经元的标记物）或血清素（5 HT）抗血清处理脑切片。这两种抗原通过过氧化物酶反应显示，但具有不同的色原，这是很容易区分的。在脊髓的运动神经元以及颅神经核的几个运动成分（包括面神经核、疑神经核和三叉神经核）中观察到NAAG样免疫反应性（NAAG-LI）和ChAT的强烈双重染色。在基底前脑胆碱能系统的胞体中，NAAG‐LI和ChAT的部分共定位是明显的，而内侧隔的胆碱能神经元仅表现出零星的NAAG‐LI染色。在蓝斑中观察到NAAG-LI和DBH完全共存。除A2细胞组几乎没有NAAG-LI外，大多数髓质区的其他去甲肾上腺素能和肾上腺素能细胞组显示出实质性的共定位。在中缝背核中，只有低百分比的肾上腺素能神经元对NAAG-LI染色。NAAG-LI和5-羟色胺的共存在中缝正中神经元中更明显，尽管大多数细胞未能显示双重染色。在中缝苍白核和大核中，与NAAG-LI的共存仍然是部分的，但涉及大百分比的多巴胺能神经元。这些结果表明一个更广泛的神经元功能NAAG除了其拟议的作用，作为推定的神经递质在一个亚组的mammatergic神经元。
An immunohistochemical technique for simultaneously visualizing two different antigens has been used to investigate the presence of the acidic dipeptide, N‐acetyl‐aspartyl‐glutamate (NAAG), in cholinergic, noradrenergic‐adrenergic, and serotonergic neurons within CNS. The brain slices were processed sequentially with purified antisera against NAAG and then monoclonal antibody against choline acetyltransferase (ChAT), a marker for cholinergic neurons, or antiserum against dopamine‐β‐hydroxylase (DBH), a marker of noradrenergic‐adrenergic neurons, or antiserum against serotonin (5HT). Both antigens were revealed by the peroxidase reaction but with different chromogens, which are easily distinguishable.An intense double staining of NAAG‐like immunoreactivity (NAAG‐LI) and ChAT was observed in the motoneurons of the spinal cord as well as in the several motor components of cranial nerve nuclei including facial, ambiguus, and trigeminal nuclei. A partial colocalization of NAAG‐LI and ChAT was evident in the perikarya of the basal forebrain cholinergic system, whereas cholinergic neurons of the medial septum exhibited only sporadic staining for NAAG‐LI. A complete coexistence of NAAG‐LI and DBH was observed in the locus coeruleus. Most of the other noradrenergic and adrenergic cell groups of the medulla region exhibited substantial co‐localization with the exception of the A2 cell group, which was virtually devoid of NAAG‐LI.In the dorsal raphe, only a low percentage of serotonergic neurons stained for NAAG‐LI. The co‐existence of NAAG‐LI and serotonin was more evident in the neurons of the median raphe, although the majority of cells failed to show double staining. In the raphe pallidus and raphe magnus, the co‐existence with NAAG‐LI remained partial but involved a large percentage of serotonergic neurons. These results suggest a more widespread neuronal function of NAAG in addition to its proposed role as putative neurotransmitter in a subgroup of glutamatergic neurons.