Microscale Thermophoresis (MST ) as a Tool for Measuring Dynamin Superfamily Protein (DSP)-Lipid Interactions.
Microscale Thermophoresis (MST ) as a Tool for Measuring Dynamin Superfamily Protein (DSP)-Lipid Interactions.
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微尺度热泳动 (MST) 作为测量动力超家族蛋白 (DSP)-脂质相互作用的工具。
DOI：
10.1007/978-1-0716-0676-6_7
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Ramachandran,Rajesh
中科院分区：
文献类型：
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作者：
Bharambe,Nikhil;Ramachandran,Rajesh
微尺度热泳（MST）是一种强大的新的基于荧光的技术，能够测量生物分子相互作用和结合亲和力（KD）。MST是表面等离子体共振（SPR）的一种无固定化替代方法，相对于等温滴定量热法（ITC）具有成本效益。在本章中，以Drp 1为例，我们首次展示了MST在确定DSP-脂质相互作用和在生理相关溶液条件下精确测量KD方面的应用。
Microscale thermophoresis (MST ) is a robust new fluorescence-based technology that enables measurement of biomolecular interactions and binding affinities (KD). MST is an immobilization-free alternative to surface plasmon resonance (SPR ) and is cost-effective relative to isothermal titration calorimetry (ITC ). In this chapter, using Drp1 as an example, we demonstrate for the first time, the application of MST to the determination of DSP–lipid interactions and the accurate measurement ofKDunder physiologically relevant solution conditions.