Probe electrospray ionization of mixture solutions using metal needles with different tip conditions
Probe electrospray ionization of mixture solutions using metal needles with different tip conditions
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使用不同尖端条件的金属针探测混合物溶液的电喷雾电离
DOI：
10.1002/sia.6558
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发表时间：
2019
影响因子：
1.7
通讯作者：
Kenzo Hiraoka
中科院分区：
文献类型：
--
作者：
Satoshi Ninomiya;Shunpei Iwamoto;Lee Chuin Chen;Kenzo Hiraoka
采用探针电喷雾质谱法测定了细胞色素c和胆固醇混合溶液的时间依赖质谱，并研究了不同针尖条件下几种金属针的PESI特性。对于细胞色素c和胆固醇的混合溶液，在0.1%甲酸甲醇/水（1/1）中，以1hz的运动频率，针尖的针刺针和直径0.5 mm的钛和不锈钢丝针产生强烈的细胞色素c和极弱的胆固醇离子信号。当频率降低，高压持续时间增加时，钛针和不锈钢针同时提供细胞色素c和胆固醇离子信号，其高强度随时间的推移而分离。
Time‐dependent mass spectra of mixture solutions of cytochrome c and cholesterol were measured by probe electrospray ionization (PESI) mass spectrometry and the PESI characteristics for several metal needles were investigated with different tip conditions. For a mixture solution of cytochrome c and cholesterol in 0.1% formic acid methanol/water (1/1) at a motion frequency of 1 Hz, an acupuncture needle with a sharp tip and 0.5‐mm‐diameter titanium and stainless‐steel wire needles gave strong cytochrome c and extremely weak cholesterol ion signals. When the frequency was lowered, and the high‐voltage duration increased, the titanium and stainless‐steel needles provided both cytochrome c and cholesterol ion signals with high intensities well separated over time.