Characterization of an amacrine cell type of the mammalian retina immunoreactive for vesicular glutamate transporter 3
Characterization of an amacrine cell type of the mammalian retina immunoreactive for vesicular glutamate transporter 3
复制标题
DOI：
10.1002/cne.10962
复制
发表时间：
2004-01-06
影响因子：
2.5
通讯作者：
Wässle, H
中科院分区：
文献类型：
--
作者：
Haverkamp, S;Wässle, H
免疫细胞化学对大鼠、小鼠和猕猴视网膜垂直切片进行抗水疱性谷氨酸转运蛋白3 （vGluT3）抗体染色，发现无痘细胞稀疏。标记的细胞在三个物种中具有相似的外观，可能代表同源类型。他们在大鼠视网膜上进行了详细的研究。vGluT3无突细胞的细静脉曲张树突形成直径约100 μ m的卷曲树突树，在内丛状层中心呈双层状。vGluT3细胞的树突被挤在两层胆碱能树突之间。vGluT3细胞在视网膜整体上的密度由周围视网膜的200/mm(2)增加到中央视网膜的400/mm(2)，约占大鼠视网膜所有无突细胞的1%。vGluT3细胞有2 - 3倍的树突重叠，它们的细胞体形成了规则的马赛克，表明它们代表了一种单一类型的无突细胞。vGluT3无分泌细胞表达甘氨酸和甘氨酸转运蛋白1 (GlyT1)，但不表达水疱性甘氨酸转运蛋白（水疱性抑制性氨基酸转运蛋白）。它们也表达谷氨酸；因此，与胆碱能无分泌细胞相比，它们有可能是“双递质”无分泌细胞。电镜观察了vGluT3细胞的突触输入。它们从带状突触的双极细胞和常规突触的其他无突细胞接收输入。通过vGluT3和钙结合蛋白CaB5的双标记切片，证实了可能与vGluT3细胞相关的双极性细胞类型。3型和5型双极细胞的轴突末端与vGluT3树突共层，vGluT3细胞可能具有ON和OFF光响应。(C) 2003 Wiley-Liss, Inc。
Immunocytochemical staining of vertical sections through rat, mouse, and macaque monkey retinae with antibodies against the vesicular glutamate transporter vesicular glutamate transporter 3 (vGluT3) showed a sparse population of amacrine cells. The labeled cells had similar appearances in the three species and probably represent homologous types. They were studied in detail in the rat retina. The thin varicose dendrites of vGluT3 amacrine cells formed a convoluted dendritic tree of approximately 100 mum in diameter that was bistratified in the center of the inner plexiform layer. The dendrites of vGluT3 cells were squeezed between the two strata of cholinergic dendrites. The density of vGluT3 cells was measured in retinal wholemounts and increased from 200/mm(2) in peripheral retina to 400/mm(2) in central retina, accounting for about 1% of all amacrine cells in the rat retina. The vGluT3 cells had a two- to threefold dendritic overlap, and their cell bodies formed a regular mosaic, suggesting they represent a single type of amacrine cell. The vGluT3 amacrine cells expressed glycine and glycine transporter 1 (GlyT1) but not the vesicular glycine transporter (vesicular inhibitory amino acid transporter). They also expressed glutamate; hence, there is the possibility that, comparable to cholinergic amacrine cells, they are "dual transmitter" amacrine cells. The synaptic input of vGluT3 cells was studied by electron microscopy. They received input from bipolar cells at ribbon synapses and from other amacrine cells at conventional synapses. The types of bipolar cells possibly involved with vGluT3 cells were demonstrated by double labeling sections for vGluT3 and the calcium-binding protein CaB5. The axon terminals of type 3 and 5 bipolar cells costratified with vGluT3 dendrites, and it is possible that vGluT3 cells have ON and OFF light responses. (C) 2003 Wiley-Liss, Inc.