Chitin synthase III:: Synthetic lethal mutants and "stress related" chitin synthesis that bypasses the CSD3/CHS6 localization pathway
Chitin synthase III:: Synthetic lethal mutants and "stress related" chitin synthesis that bypasses the CSD3/CHS6 localization pathway
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DOI：
10.1073/pnas.96.20.11206
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发表时间：
1999-09-28
影响因子：
11.1
通讯作者：
Robbins, PW
中科院分区：
文献类型：
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作者：
Osmond, BC;Specht, CA;Robbins, PW
我们筛选了具有几丁质合成酶基因CHS3缺失的合成致死突变体的酵母菌菌株。除了发现主要细胞壁相关基因（如FKS1（葡聚糖合成酶））的突变和任何高尔基糖基化复合体基因（MNN9家族）的突变在chs3缺失中是致命的外，我们还发现Srv2p（一个双功能调节基因）的突变在chs3缺失中是致命的。在对fks1-几丁质合成酶3相互作用的扩展研究中，我们惊奇地发现CSD3/CHS6的缺失对fks1并不致命，事实上，缺乏Csd3p/Chs6p并没有降低fks1突变体中与应激相关的高水平几丁质。CSD3/CHS6是Chs3p在体内传递和活性所必需的基因。这一发现表明“应激反应”几丁质合成通过另一种Chs3p靶向途径进行。
We screened Saccharomyces strains for mutants that are synthetically lethal with deletion of the major chitin synthase gene CHS3. In addition to finding, not surprisingly, that mutations in major cell wall-related genes such as FKS1 (glucan synthase) and mutations in any of the Golgi glycosylation complex genes (MNN9 family) are lethal in combination with chs3 Delta, we found that a mutation in Srv2p, a bifunctional regulatory gene, is notably lethal in the chs3 deletion. In extending studies of fks1-chitin synthase 3 interactions, we made the surprising discovery that deletion of CSD3/CHS6, a gene normally required for Chs3p delivery and activity in vivo, was not lethal with fks1 and, in fact, that lack of Csd3p/Chs6p did not decrease the high level of stress-related chitin made in the fks1 mutant. This finding suggests that "stress response" chitin synthesis proceeds through an alternate Chs3p targeting pathway.