Seed isoflavone profiling of 1168 soybean accessions from major growing ecoregions in China
Seed isoflavone profiling of 1168 soybean accessions from major growing ecoregions in China
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来自中国主要生长生态区的 1168 个大豆品种的种子异黄酮分析
DOI：
10.1016/j.foodres.2019.108957
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发表时间：
2020-04-01
影响因子：
8.1
通讯作者：
Sun, Junming
中科院分区：
文献类型：
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作者：
Azam, Muhammad;Zhang, Shengrui;Sun, Junming
大豆异黄酮是一种对人体健康有益的次生代谢物，对人体健康有很大的影响。在两年多的时间里，我们对来自中国不同生态区的1 168份大豆种质的种子异黄酮组分进行了分析。我们观察到异黄酮类成分在不同来源、不同类型、不同生长年限和不同生态区之间存在显著差异。大豆种质的总异黄酮含量在745mgg(-1)到5253.98 mgg(-1)之间，相差7倍。黄淮海谷区种质的TIF平均含量最高，为2689.27 mU g g(-1)，其次是南方和北方种质，分别为2518.91 mU g-1和1942.78 mU g-1。根据较高的TIF浓度(4024.74~5253.98 mgg-1)，鉴定出35份大豆资源为优良种质。两两相关分析表明，单株异黄酮与TIF浓度呈显著正相关。丙二酰大豆苷和丙二酰染料木苷与TIF浓度的相关性最高(r=0.90和r=0.92)，而乙酰大豆苷的相关性最低。主要异黄酮组分与纬度和经度呈显著负相关，表明地理来源影响其种子异黄酮组分组成。主成分分析表明，大豆异黄酮苷和丙二酸苷是大豆种质的主要鉴别成分。本研究论证了大豆种子异黄酮浓度在中国主要生态区的地理分布。鉴定出的高、低TIF含量的大豆材料，既是工业上理想的材料，也可以作为亲本选育出种子中异黄酮量和组成都得到改善的大豆品系。
Soybean (Glycine max L Merrill) isoflavones are secondary metabolites of great interest because of their beneficial impact on human health. We profiled the seed isoflavone composition of 1168 soybean accessions collected from diverse ecoregions of China in three locations over two years. We observed significant differences in isoflavone content among the accessions, accession types, years of growth and ecoregions of origin. Total isoflavone (TIF) concentration of the soybean accessions ranged from 745 mu g g(-1) to 5253.98 mu g g(-1), which represents a 7-fold difference. The highest mean TIF concentration (2689.27 mu g g(-1)) was observed in the Huang Huai Hai Valley Region (HR) accessions, followed by accessions from the Southern Region (SR) and Northern Region (NR) with TIF concentration of 2518.91 mu g g(-1) and 1942.78 mu g g(-1), respectively. Thirty-five accessions were identified as elite soybean resources based on their higher TIF concentration (4024.74 mu g g(-1) to 5253.98 mu g g(-1)). Pairwise correlation analysis showed significant positive correlations between individual isoflavones and TIF concentrations. Malonyldaidzin and malonylgenistin showed the highest correlations with TIF concentration (r = 0.90 and r = 0.92, respectively), whereas acetyldaidzin showed the lowest correlation. The main isoflavone components had significant negative correlations with latitude and longitude, indicating that the geographical origin of the accessions influenced their seed isoflavone composition. Based on principal component analysis, glycosides and malonylglycosides of isoflavones were the major discriminative components for the soybean accessions. The present study demonstrated the geographical distribution of soybean seed isoflavone concentrations across the main ecoregion of China. The identified soybean accessions with both high and low TIF concentrations, which are desirable materials for industrial uses and could also be used as parents to breed soybean lines with improved isoflavone quantity and composition in the seeds.