Exposure to 60 Hz magnetic field can affect membrane proteins and membrane potential in human cancer cells
Exposure to 60 Hz magnetic field can affect membrane proteins and membrane potential in human cancer cells
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DOI：
10.1080/15368378.2021.1958340
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发表时间：
2021-08-15
影响因子：
1.7
通讯作者：
Kakikawa, Makiko
中科院分区：
文献类型：
--
作者：
Hayashi, Seiya;Kakikawa, Makiko
实验数据支持极低频磁场（ELF-MF）可以影响细胞膜的假设。由于我们之前的研究表明，磁场改变了细胞膜的通透性，因此在本研究中，我们以细胞膜为研究对象，研究了60hz， 50mt磁场对膜电位和膜蛋白的影响。体外培养的3种人癌细胞系A549、MES-SA和MES-SA/Dx5在ELF-MF作用下膜电位升高。当暴露于MF和抗癌药物时，A549和MES-SA细胞的膜电位发生了变化，但在多重耐药细胞MES-SA/Dx5中没有变化。我们使用DiBAC(4)(3)染料增强荧光与疏水位点结合，检测MF是否对从培养的A549细胞中提取的膜蛋白有影响。在MF暴露10分钟后观察到荧光增加，这表明膜蛋白上疏水位点的结构发生了变化，更容易与探针染料结合。暴露于MF中240分钟后，检测到荧光减少。结果表明，60 Hz、50 mT的MF可引起培养癌细胞膜电位及提取的膜蛋白构象的变化，且影响随暴露时间的不同而不同。
The experimental data support the hypothesis that extremely low frequency magnetic field (ELF-MF) can affect cell membranes. Since our previous studies suggested that MF changes the permeability of cell membrane, in this study we focused on the cell membrane and investigated the effect of 60 Hz, 50 mT MF on the membrane potential and membrane proteins. The membrane potentials of three cultured human cancer cell lines, A549, MES-SA, and MES-SA/Dx5, were increased by exposure to ELF-MF. When exposed to MF and an anticancer drug, changes in the membrane potentials were detected in A549 and MES-SA cells, but not in the multi drug-resistant cells, MES-SA/Dx5. We examined whether MF has an influence on the membrane proteins extracted from cultured A549 cells, using DiBAC(4)(3) dye enhanced fluorescence binding to a hydrophobic site. The increase in fluorescence observed following MF exposure for 10 min indicated that the structure of the hydrophobic site on the membrane proteins changed and became more likely to bind the probe dye. A decrease in fluorescence was detected following exposure to MF for 240 min. These results indicated that 60 Hz, 50 mT MF causes changes in the membrane potential of cultured cancer cells and the conformation of membrane proteins extracted from cultured cancer cells, and has different effects depending on the exposure time.