Functional development of the vagal and glossopharyngeal nerve-related nuclei in the embryonic rat brainstem: optical mapping with a voltage-sensitive dye.
Functional development of the vagal and glossopharyngeal nerve-related nuclei in the embryonic rat brainstem: optical mapping with a voltage-sensitive dye.
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胚胎大鼠脑干中迷走神经和舌咽神经相关核团的功能发育：使用电压敏感染料进行光学测绘。
DOI：
10.1016/j.neuroscience.2011.06.019
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发表时间：
2011
期刊：
影响因子：
3.3
通讯作者：
K.
中科院分区：
文献类型：
--
作者：
Momose-Sato,Y.;Nakamori;T. and Sato;K.
我们使用快速电压敏感染料 NK2761 进行多位点光学记录，研究了胚胎大鼠脑干中迷走神经 (N. X) 和舌咽神经 (N. IX) 相关核团的功能组织，并比较了它们的发育和空间分布模式。从 E13-E16 大鼠胚胎中解剖出附有 N.X 和 N.IX 的完整脑干制剂，并由 N.X/N 诱发电反应。从染色制剂的许多位点光学记录 IX 刺激。我们分析了光波形并分离了分别对应于逆向/顺向动作电位和兴奋性突触后电位（EPSP）的快速和慢速光信号。我们构建了信号幅度的等高线图，并识别了 N.X 和 N.IX 的运动和感觉核。在 N.X 相关运动核（迷走神经背侧运动核：DMNV）中，快信号以多峰模式分布，表明神经元和/或其活动在早期发育阶段在运动核内分布不均匀。在感觉核（孤束核：NTS）中，首先从正常生理溶液中的 E15 中检测到 N.X 和 N.IX 的 EPSP。 N.IX相关的NTS与N.X相关的NTS部分重叠，但是这两个神经之间的峰值位置不同。本研究获得的结果表明，N.X 和 N.IX 相关核的功能组织随着胚胎大鼠脑干的发育而动态变化。
We investigated functional organization of the vagus nerve (N. X)- and glossopharyngeal nerve (N. IX)-related nuclei in the embryonic rat brainstem and compared their development and spatial distribution patterns, using multiple-site optical recording with a fast voltage-sensitive dye, NK2761. Intact brainstem preparations with N. X and N. IX attached were dissected from E13-E16 rat embryos, and electrical responses evoked by N. X/N. IX stimulation were optically recorded from many loci of the stained preparations. We analyzed optical waveforms and separated fast and slow optical signals corresponding to the antidromic/orthodromic action potentials and the excitatory postsynaptic potentials (EPSPs), respectively. We constructed contour line maps of signal amplitudes and identified motor and sensory nuclei of N. X and N. IX. In the N. X-related motor nucleus (the dorsal motor nucleus of the vagus nerve: DMNV), the fast signals were distributed in multiple-peak patterns, suggesting that the neurons and/or their activity are not distributed uniformly within the motor nuclei at early developmental stages. In the sensory nucleus (the nucleus of the tractus solitarius: NTS), the EPSPs were first detected from E15 in normal physiological solution for both N. X and N. IX. The N. IX-related NTS partially overlapped with the N. X-related NTS, but the peak locations were different between these two nerves. The results obtained in this study suggest that functional organization of the N. X- and N. IX-related nuclei changes dynamically with development in the embryonic rat brainstem.