Identification and characterization of MicroRNAs expressed in the mouse eye
Identification and characterization of MicroRNAs expressed in the mouse eye
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DOI：
10.1167/iovs.06-0866
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发表时间：
2007-02-01
影响因子：
4.4
通讯作者：
Banfi, Sandro
中科院分区：
文献类型：
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作者：
Karali, Marianthi;Peluso, Ivana;Banfi, Sandro
目的. microRNA（miRNAs）是一类内源性小RNA，通过与信使RNA的3'非翻译区（UTR）靶位点结合，在转录后负调控基因表达。虽然已经发现它们调节几个器官和组织的发育和生理过程，但它们在眼睛转录组中的作用完全未知。本研究通过高分辨率表达分析寻找在小鼠眼内显著表达的miRNAs，以了解其在哺乳动物眼内的相关功能。通过RNA原位杂交（ISH），使用LNA修饰的寡核苷酸探针，分析了小鼠胚胎和出生后眼睛中miRNA的时空定位。7种miRNAs在眼睛中以不同的和部分重叠的模式表达，这可能反映了它们在控制视网膜以及其他眼部结构的细胞分化中的作用。大多数眼睛表达的miRNA与主要来自眼睛cDNA文库的转录物重叠或在其附近。我们发现这些转录本与其对应的miRNAs在细胞内的分布非常相似，提示miRNAs可能与其宿主基因具有共同的表达调控元件。这些数据提供了眼睛富集的miRNA表达的详细表征。了解miRNAs的时空分布是确定其靶点并最终阐明其在眼睛发育和功能中的生物学作用的重要一步。
PURPOSE. MicroRNAs ( miRNAs) are a class of small, endogenous RNAs that negatively regulate gene expression post-transcriptionally by binding to target sites in the 3' untranslated region (UTR) of messenger RNAs. Although they have been found to regulate developmental and physiological processes in several organs and tissues, their role in the eye transcriptome is completely unknown. This study was conducted to gain understanding of their eye-related function in mammals, by looking for miRNAs significantly expressed in the mouse eye by means of high-resolution expression analysis.METHODS. The spatiotemporal localization of miRNAs was analyzed in the murine embryonic and postnatal eye by RNA in situ hybridization ( ISH) using LNA-modified oligonucleotide probes.RESULTS. Seven miRNAs were expressed in the eye with diverse and partially overlapping patterns, which may reflect their role in controlling cell differentiation of the retina as well as of other ocular structures. Most eye-expressed miRNAs overlap with or are in the near vicinity of transcripts derived predominantly from eye cDNA libraries. We found that these transcripts share very similar cellular distribution with their corresponding miRNAs, suggesting that miRNAs may share common expression regulatory elements with their host genes.CONCLUSIONS. The data provide a detailed characterization of expression of eye-enriched miRNAs. Knowledge of the spatiotemporal distribution of miRNAs is an essential step toward the identification of their targets and eventually the elucidation of their biological role in eye development and function.