MATHEMATICAL TREATMENT OF ELECTROPHORETICALLY MEDIATED MICROANALYSIS
MATHEMATICAL TREATMENT OF ELECTROPHORETICALLY MEDIATED MICROANALYSIS
复制标题
DOI：
10.1021/ac00067a018
复制
发表时间：
1993-10-01
影响因子：
7.4
通讯作者：
REGNIER, FE
中科院分区：
文献类型：
--
作者：
HARMON, BJ;PATTERSON, DH;REGNIER, FE
介绍了反应化学分析中的一个新概念，并从理论上进行了阐述。通过利用带电物质之间的电泳迁移率的可变性，化学试剂的空间上不同的区域可以在施加的电场的影响下被电泳地合并。电泳介导微量分析（EMMA）利用这种现象作为利用毛细管电泳系统进行化学分析的基础。EMMA按照基于反应的分析所需的四个阶段进行描述：（1）分析物和分析试剂计量;（2）反应启动;（3）反应条件和产物形成的控制;（4）检测其产生或消耗指示感兴趣的分析物的浓度或数量的物质。该方法通过乙醇脱氢酶将乙醇酶促氧化为乙醛来说明，同时在340 nm处监测NAD+还原为NADH。底物和酶测定的实验结果表明，同意所提出的理论。
A new concept in reaction-based chemical analysis is introduced and theoretically described. By utilization of the variability in electrophoretic mobilities among charged species, spatially distinct zones of chemical reagents can be electrophoretically merged under the influence of an applied electric field. Electrophoretically mediated microanalysis (EMMA) exploits this phenomenon as a basis for chemical analysis utilizing capillary electrophoretic systems. EMMA is described in terms of the four stages required for reaction-based analysis: (1) analyte and analytical reagent metering; (2) initiation of reaction; (3) control of reaction conditions and product formation; (4) detection of species whose production or depletion is indicative of the concentration or quantity of the analyte of interest. The method is illustrated by the enzymatic oxidation of ethanol to acetaldehyde by alcohol dehydrogenase with the concurrent reduction of NAD+ to NADH monitored at 340 nm. Experimental results for both substrate and enzyme determinations are shown to agree with the presented theory.