Plasticity of xyloglucan composition in bean (Phaseolus vulgaris)-cultured cells during habituation and dehabituation to lethal concentrations of dichlobenil.
Plasticity of xyloglucan composition in bean (Phaseolus vulgaris)-cultured cells during habituation and dehabituation to lethal concentrations of dichlobenil.
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DOI：
10.1093/mp/ssq011
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发表时间：
2010-05
期刊：
影响因子：
27.5
通讯作者：
Ana Alonso-Simón;Lutz Neumetzler;P. García-Angulo;A. Encina;J. Acebes;J. Álvarez;Takahisa Hayashi
中科院分区：
文献类型：
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作者：
Ana Alonso-Simón;Lutz Neumetzler;P. García-Angulo;A. Encina;J. Acebes;J. Álvarez;Takahisa Hayashi
已经习惯于在致命浓度(12μM)的2，6-二氯苯腈(双氯苯尼或纤维素生物合成抑制剂)中生长的豆类细胞，其细胞壁中的纤维素含量减少。木葡聚糖与纤维素结合并与之一起构成植物细胞壁的主要负载网络，在习惯性细胞中也被描述为减少，但除了其丰度外，纤维素的变化是否会影响木葡聚糖的结构尚未得到分析。木葡聚糖专一性内切酶(XEG)和胞内纤维素酶的碎裂分析表明，对DCB的习惯性改变了木聚糖的精细结构，即葡聚糖骨架上附着在半乳糖-木糖基残基上的岩藻糖基减少。在去除除草剂后，木葡聚糖可恢复其岩藻糖基残基。此外，一些纤维素低聚糖只能在XEG和内切酶消化的驯化细胞的木聚糖中检测到，对应于与半纤维素共价结合或共沉淀的葡聚糖。这些结果表明，细胞壁的结构柔性在一定程度上依赖于木聚糖组分的可塑性，为该领域的进一步研究开辟了新的前景。
Bean cells that have been habituated to grow in a lethal concentration (12 μM) of 2,6-dichlorobenzonitrile (dichlobenil or DCB, a cellulose biosynthesis inhibitor) are known to have decreased cellulose content in their cell walls. Xyloglucan, which is bound to cellulose and together with it forms the main loading network of plant cell walls, has also been described to decrease in habituated cells, but whether the change on cellulose affects the xyloglucan structure besides its abundance has not been analyzed. Fragmentation analysis with xyloglucan-specific endoglucanase (XEG) and endocellulase revealed that habituation to DCB caused a change in the fine structure of xyloglucan, namely a decrease in fucosyl residues attached to the galactosyl-xylosyl residues along the glucan backbone. After the removal of herbicide from the medium (dehabituated cells), xyloglucan recovered its fucosyl residues. In addition, some cello-oligosaccharides could be detected only in habituated cells' xyloglucan digested by XEG and endocellulase, corresponding to a glucan covalently bound or co-precipitated with the hemicelluloses. These results show that structural flexibility of cell walls relies in part on the plasticity of xyloglucan composition and opens up new perspectives to further research in this field.