4D Printing of Engineered Living Materials
4D Printing of Engineered Living Materials
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DOI：
10.1002/adfm.202106843
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发表时间：
2021-10-15
影响因子：
19
通讯作者：
Ware, Taylor H.
中科院分区：
文献类型：
--
作者：
Rivera-Tarazona, Laura K.;Shukla, Tarjani;Ware, Taylor H.
在此，报道了一种使用直接墨写印刷来制造工程活性材料（ELM）的方法，所述工程活性材料通过响应于特定分子而经历编程的形状变化来响应。通过整合仅在特定生物分子存在下增殖的基因工程酵母，赋予ELMs刺激响应性。这种增殖反过来导致ELM响应于该生物分子的形状变化。这些ELM是通过共打印含有多种酵母菌株的生物墨水制造的。在局部，细胞增殖导致材料的可控形状变化，导致体积增加高达370%。在全球范围内，打印的3D结构包含体积增加的材料区域和在给定条件下不增加的区域，从而导致响应于目标氨基酸和核苷酸的形式的可编程变化。最后，这种印刷方法被应用于设计一个基于药物的药物递送系统，用于响应于特定的生物分子按需递送模型药物。
Herein, a method that uses direct-ink-write printing to fabricate engineering living materials (ELMs) that respond by undergoing a programmed shape change in response to specific molecules is reported. Stimuli-responsiveness is imparted to ELMs by integrating genetically engineered yeast that only proliferate in the presence of specific biomolecules. This proliferation, in turn, leads to a shape change in the ELM in response to that biomolecule. These ELMs are fabricated by coprinting bioinks that contain multiple yeast strains. Locally, cellular proliferation leads to controllable shape change of the material resulting in up to a 370% increase in volume. Globally, the printed 3D structures contain regions of material that increase in volume and regions that do not under a given set of conditions, leading to programmable changes in form in response to target amino acids and nucleotides. Finally, this printing method is applied to design a reservoir-based drug delivery system for the on-demand delivery of a model drug in response to a specific biomolecule.