Clinical, histopathological, and immunological responses of ponies to Ehrlichia sennetsu and subsequent Ehrlichia risticii challenge
Clinical, histopathological, and immunological responses of ponies to Ehrlichia sennetsu and subsequent Ehrlichia risticii challenge
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小马对 sennetsu 埃里希体和随后的埃里希体攻击的临床、组织病理学和免疫学反应
DOI：
10.1128/iai.56.11.2960-2966.1988
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发表时间：
1988
影响因子：
3.1
通讯作者：
F. Andrews
中科院分区：
文献类型：
--
作者：
Y. Rikihisa;C. Pretzman;G. Johnson;S. Reed;S. Yamamoto;F. Andrews
Ehrlichia ristii 与 E. sennetsu 具有密切的抗原关系。在间接荧光抗体（IFA）测试中，实验性感染了波托马克马热病原体 E. risticii 的小马血清与人类病原体 E. sennetsu 发生反应，而通过 IFA 测试和体外感染细胞的免疫铁蛋白标记，人类 E. sennetsu 恢复期血清与 E. sennetsu 发生反应。两匹小马静脉注射了活的仙乃石斑鱼，没有出现临床疾病。随后注射活 E. sennetsu 并没有出现临床疾病。随后注射活的里斯提氏小马也不会诱发任何疾病，相比之下，两只对照小马在未事先接触仙奈特马的情况下注射了里斯提氏小马。两名对照均出现发热、厌食、抑郁、脱水和腹泻，这些都是波托马克马热的典型临床症状，尸检时有肠炎和淋巴结组织细胞增多症的特征性病变。暴露于 E. sennetsu 的小马具有正常的胃肠道形态和淋巴结增生。在 E. ristii 攻击之前用 E. sennetsu 引发的小马产生了高滴度的免疫球蛋白 G 抗体，通过 IFA 测试，该抗体对 E. sennetsu 和 E. ristii 抗原都发生反应。在随后用 E. ristii 攻击之前，对先用 E. sennetsu 引发的小马收集的血清进行蛋白质印迹分析，最显着的抗原性多肽存在于两种埃里希体属物种的裂解物中，并且具有 44 千道尔顿的表观分子质量。在用单独注射了 E. ristii 的小马血清进行的蛋白质印迹中，该条带并不明显。因此，注射 E. sennetsu 可以保护小马免受注射 E. ristii 引起的疾病的临床和病理表现。通过 IFA 测试发现两种生物体的免疫交叉反应性以及两种生物体共有的 44 千道尔顿抗原的强烈免疫识别可能与针对 E. risticii 的保护性免疫的发展有关。
Ehrlichia risticii has a close antigenic relationship to E. sennetsu. Sera of ponies experimentally infected with E. risticii, the etiologic agent of Potomac horse fever, consistently reacted with E. sennetsu, a human pathogen, in indirect fluorescent-antibody (IFA) testing, while human E. sennetsu convalescent serum reacted with E. risticii by IFA testing and immunoferritin labeling of cells infected in vitro. Two ponies injected intravenously with live E. sennetsu did no develop clinical illness. Subsequent injection with live E. sennetsu did not develop clinical illness. Subsequent injection with live E. risticii also did not induce any disease, in contrast to two control ponies given E. risticii without prior exposure to E. sennetsu. Both controls developed fever, anorexia, depression, dehydration, and diarrhea, which are typical clinical signs of Potomac horse fever, and had characteristic lesions of enteritis and lymph node histiocytosis at postmortem examination. E. sennetsu-exposed ponies had normal gastrointestinal morphologies and lymph node hyperplasia. Ponies primed with E. sennetsu before E. risticii challenge developed high titers of immunoglobulin G antibody which reacted against both E. sennetsu and E. risticii antigens by IFA testing. The most prominent antigenic polypeptide in Western (immuno-) blot analysis of sera collected from ponies primed with E. sennetsu before subsequent challenge with E. risticii was present in lysates of both Ehrlichia species and had an apparent molecular mass of 44 kilodaltons. This band was not prominent in Western blots performed with sera of ponies injected with E. risticii alone. Thus, injection of E. sennetsu protects ponies from clinical and pathological manifestations of the disease induced by injection with E. risticii. Immunologic cross-reactivity of the two organisms with IFA testing and strong immunologic recognition by ponies of the 44-kilodalton antigen common to the two organisms may be related to the development of protective immunity against E. risticii.