Preliminary investigation of a hypertonic saline nasal rinse as a hygienic intervention in dairy workers.
Preliminary investigation of a hypertonic saline nasal rinse as a hygienic intervention in dairy workers.
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高渗盐水鼻冲洗作为奶牛工人卫生干预的初步研究。
DOI：
10.1080/15459624.2022.2137297
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发表时间：
2023
影响因子：
2
通讯作者：
Schaeffer,JoshuaW
中科院分区：
文献类型：
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作者：
Erlandson,Grant;Magzamen,Sheryl;Sharp,JuliaL;Mitra,Sanchayita;Jones,Kenneth;Poole,JillA;Bradford,Mary;Nonnenmann,Matthew;Reynolds,StephenJ;Schaeffer,JoshuaW
畜牧业工人经历了生物气雾剂引起的呼吸系统疾病的负担增加，包括鼻窦炎的高流行率。奶制品作业产生的生物气雾剂跨越可吸入颗粒(0-100 μm)，含有内毒素等细菌成分。已知空气动力学直径在10到100 μm之间的颗粒沉积在鼻咽区，并可能影响上呼吸道。我们评估了高渗盐水鼻腔灌洗在减少大容量乳品作业中乳品工人炎症反应方面的有效性。收集每个参与者连续5天的可吸入个人呼吸区样本和轮班前/后鼻腔冲洗样本。治疗组(n = 5)给予高渗盐水，对照组(n = 5)给予生理盐水。分别用重量法和酶法对个人呼吸区样本进行了颗粒物浓度和内毒素分析。用多重分析方法从鼻腔灌洗样本中检测促炎和抗炎细胞因子(即IL-8、IL-10和肿瘤坏死因子-α)。可吸入粉尘浓度范围为0.15至1.9 毫克/立方米。治疗组促炎症和抗炎细胞因子，特别是IL-6、IL-8和IL-10的浓度均显著高于对照组(P<； 分别为0.02、P<； 和P<； 分别为0.02、0.04和0.01)。对IL-10抗炎作用的进一步分析表明，给予高渗盐水与IL-10的产生呈正相关。这项初步研究表明，高渗盐水鼻腔灌洗成功地上调了抗炎细胞因子，以支持更大规模的干预性研究。
Livestock workers experience an increased burden of bioaerosol-induced respiratory disease including a high prevalence of rhinosinusitis. Dairy operations generate bioaerosols spanning the inhalable size fraction (0–100 μm) containing bacterial constituents such as endotoxin. Particles with an aerodynamic diameter between 10 and 100 μm are known to deposit in the nasopharyngeal region and likely affect the upper respiratory tract. We evaluated the effectiveness of a hypertonic saline nasal lavage in reducing inflammatory responses in dairy workers from a high-volume dairy operation. Inhalable personal breathing zone samples and pre-/post-shift nasal lavage samples from each participant over five consecutive days were collected. The treatment group (n = 5) received hypertonic saline while the control group (n = 5) received normotonic saline. Personal breathing zone samples were analyzed for particulate concentrations and endotoxin using gravimetric and enzymatic methods, respectively. Pro- and anti-inflammatory cytokines (i.e., IL-8, IL-10, and TNF-α) were measured from nasal lavage samples using a multiplex assay. Inhalable dust concentrations ranged from 0.15 to 1.9 mg/m3. Concentrations of both pro- and anti-inflammatory cytokines, specifically IL-6, IL-8, and IL-10, were significantly higher in the treatment group compared to the control group (p< 0.02,p< 0.04, andp< 0.01, respectively). Further analysis of IL-10 anti-inflammatory indicates a positive association between hypertonic saline administration and IL-10 production. This pilot study demonstrates that hypertonic saline nasal lavages were successful in upregulating anti-inflammatory cytokines to support larger interventional studies.