Human Herpesvirus 6 Detection in Alzheimer's Disease Cases and Controls across Multiple Cohorts
Human Herpesvirus 6 Detection in Alzheimer's Disease Cases and Controls across Multiple Cohorts
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DOI：
10.1016/j.neuron.2019.12.031
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发表时间：
2020-03-18
期刊：
影响因子：
16.2
通讯作者：
Jacobson, Steven
中科院分区：
文献类型：
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作者：
Allnutt, Mary Alice;Johnson, Kory;Jacobson, Steven
病毒感染与阿尔茨海默病（AD）之间的相互作用长期以来一直是一个令人感兴趣的领域，但证明因果关系一直难以捉摸。最近的几项研究重新引发了关于疱疹病毒，特别是人类疱疹病毒6 （HHV-6）在阿尔茨海默病中的作用的争论。我们使用(1)RNA测序（RNA-seq）数据集和(2)从AD和非AD对照脑中提取的DNA样本，在三个独立的AD脑库中筛选HHV-6检测。RNA-seq数据筛选了超过25,000种微生物（包括118种人类病毒）的病原分类参考，而DNA样本则检测了对HHV-6A和HHV-6B的PCR反应性。与对照组相比，HHV-6对AD大脑的特异性较低，而其他病毒，如EBV和巨细胞病毒（CMV）的检测水平相当。这些直接的病毒检测方法并不表明HHV-6和AD之间存在关联。
The interplay between viral infection and Alzheimer's disease (AD) has long been an area of interest, but proving causality has been elusive. Several recent studies have renewed the debate concerning the role of herpesviruses, and human herpesvirus 6 (HHV-6) in particular, in AD. We screened for HHV-6 detection across three independent AD brain repositories using (1) RNA sequencing (RNA-seq) datasets and (2) DNA samples extracted from AD and non-AD control brains. The RNA-seq data were screened for pathogens against taxon references from over 25,000 microbes, including 118 human viruses, whereas DNA samples were probed for PCR reactivity to HHV-6A and HHV-6B. HHV-6 demonstrated little specificity to AD brains over controls by either method, whereas other viruses, such as EpsteinBarr virus (EBV) and cytomegalovirus (CMV), were detected at comparable levels. These direct methods of viral detection do not suggest an association between HHV-6 and AD.