Enhanced proliferation of primary rat type II pneumocytes by Jaagsiekte sheep retrovirus envelope protein
Enhanced proliferation of primary rat type II pneumocytes by Jaagsiekte sheep retrovirus envelope protein
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DOI：
10.1016/j.virol.2011.01.022
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发表时间：
2011-04-10
期刊：
影响因子：
3.7
通讯作者：
Fan, Hung
中科院分区：
文献类型：
--
作者：
Johnson, Chassidy;Jahid, Sohail;Fan, Hung
Jaagsiekte 绵羊逆转录病毒 (JSRV) 是绵羊传染性肺癌的病原体。包膜蛋白 (Env) 是癌基因，因为它可以转化培养物中的细胞系并在动物中诱导肿瘤，但转化机制尚不清楚，因为不存在在分化的 II 型肺细胞中进行转化测定的系统。在这项研究中，我们报告了原代大鼠 II 型肺细胞在有利于 II 型肺细胞标记物长时间表达的条件下的培养。与载体对照样品相比，表达 Env 的培养物形成了更多的菌落，其尺寸更大且存活时间更长。培养中保留时间较长的细胞被证实源自 II 型肺细胞，因为它们表达表面活性蛋白 C、细胞角蛋白，表现出碱性磷酸酶活性，并且尼罗红呈阳性。该系统将有助于研究 JSRV Env 的转化目标。 (C) 2011 Elsevier Inc. 保留所有权利。
Jaagsiekte sheep retrovirus (JSRV) is the causative agent of a contagious lung cancer in sheep. The envelope protein (Env) is the oncogene, as it can transform cell lines in culture and induce tumors in animals, although the mechanisms for transformation are not yet clear because a system to perform transformation assays in differentiated type II pneumocytes does not exist. In this study we report culture of primary rat type II pneumocytes in conditions that favor prolonged expression of markers for type II pneumocytes. Env-expressing cultures formed more colonies that were larger in size and were viable for longer periods of time compared to vector control samples. The cells that remained in culture longer were confirmed to be derived from type II pneumocytes because they expressed surfactant protein C, cytokeratin, displayed alkaline phosphatase activity and were positive for Nile red. This system will be useful to study JSRV Env in the targets of transformation. (C) 2011 Elsevier Inc. All rights reserved.