Social propinquity in rodents as measured by tube cooccupancy differs between inbred and outbred genotypes
Social propinquity in rodents as measured by tube cooccupancy differs between inbred and outbred genotypes
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DOI：
10.1073/pnas.1703477114
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发表时间：
2017-05-23
影响因子：
11.1
通讯作者：
Mogil, Jeffrey S.
中科院分区：
文献类型：
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作者：
Tuttle, Alexander H.;Tansley, Shannon;Mogil, Jeffrey S.
现有的社会互动分析是次优的，没有测量接近度，啮齿动物保持身体接近的趋势。这些测定普遍使用近交系小鼠品系和置于近交系遗传背景上的突变进行。我们开发了自动管共占试验（TCOT）的基础上propinquity，自由移动的啮齿动物保持密切的物理接近的趋势，并评估TCOT行为的各种基因型和社会和环境条件。在远交系小鼠和大鼠，熟悉决定愿意共同占用管，与兄弟姐妹和/或笼中的两性表现出较高的共同占用行为比陌生人。随后的测试使用多个基因型显示，近交系的兄弟姐妹不同居率高于陌生人，在显着的对比远交和再衍生的野生小鼠。具有“自闭症样”表型（Fmr 1（-/y）和Eif 4 e Ser 209 Ala）的突变小鼠品系显示出显著降低的共占有率。
Existing assays of social interaction are suboptimal, and none measures propinquity, the tendency of rodents to maintain close physical proximity. These assays are ubiquitously performed using inbred mouse strains and mutations placed on inbred genetic backgrounds. We developed the automatable tube cooccupancy test (TCOT) based on propinquity, the tendency of freely mobile rodents to maintain close physical proximity, and assessed TCOT behavior on a variety of genotypes and social and environmental conditions. In outbred mice and rats, familiarity determined willingness to cooccupy the tube, with siblings and/or cagemates of both sexes exhibiting higher cooccupancy behavior than strangers. Subsequent testing using multiple genotypes revealed that inbred strain siblings do not cooccupy at higher rates than strangers, in marked contrast to both outbred and rederived wild mice. Mutant mouse strains with "autistic-like" phenotypes (Fmr1(-/y) and Eif4e Ser209Ala) displayed significantly decreased cooccupancy.