Polarity switching mass spectrometry imaging of healthy and cancerous hen ovarian tissue sections by infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI).
Polarity switching mass spectrometry imaging of healthy and cancerous hen ovarian tissue sections by infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI).
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DOI：
10.1039/c5an01513h
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发表时间：
2016-01-21
期刊：
影响因子：
--
通讯作者：
Muddiman DC
中科院分区：
文献类型：
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作者：
Nazari M;Muddiman DC
质谱成像（MSI）是用于监测生物组织切片中分析物的空间分布和丰度的快速发展的领域。它允许以无标记的方式直接和同时分析数百种不同的化合物。为了获得全面的代谢物和脂质数据，开发并优化了使用红外基质辅助激光解吸电喷雾电离（IR-MALDESI）的极性切换MSI方法，其中电喷雾极性从一个体素到下一个体素交替。使用该方法分析健康和癌性卵巢母鸡组织切片。识别每个组织切片内不同代谢物和脂质的分布和相对丰度，并揭示两者之间的差异。此外，使用质谱的概念，如光谱准确性和硫计数的非靶向方法中的分析物的置信鉴定的效用进行了讨论。IR-MALDESI极性转换质谱成像揭示了母鸡卵巢癌组织中脂质分布的差异。
Mass spectrometry imaging (MSI) is a rapidly evolving field for monitoring the spatial distribution and abundance of analytes in biological tissue sections. It allows for direct and simultaneous analysis of hundreds of different compounds in a label-free manner. In order to obtain a comprehensive metabolite and lipid data, a polarity switching MSI method using infrared matrix assisted laser desorption electrospray ionization (IR-MALDESI) was developed and optimized where the electrospray polarity was alternated from one voxel to the next. Healthy and cancerous ovarian hen tissue sections were analyzed using this method. Distribution and relative abundance of different metabolites and lipids within each tissue section were discerned, and differences between the two were revealed. Additionally, the utility of using mass spectrometry concepts such as spectral accuracy and sulfur counting for confident identification of analytes in an untargeted method are discussed. IR-MALDESI polarity switching mass spectrometry imaging reveals differences in lipid distribution in hen ovarian cancer tissue.