Antibacterial Synergy of Silver Nanoparticles with Gentamicin and Chloramphenicol against Enterococcus faecalis.
Antibacterial Synergy of Silver Nanoparticles with Gentamicin and Chloramphenicol against Enterococcus faecalis.
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DOI：
10.4103/pm.pm_120_17
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发表时间：
2018-01
影响因子：
0.7
通讯作者：
Kaushik S
中科院分区：
文献类型：
--
作者：
Katva S;Das S;Moti HS;Jyoti A;Kaushik S
粪肠球菌 (Ef) 是一种与医院获得性感染相关的多重耐药病原菌。 Ef 与多种传染病有关。它通常感染具有周末免疫系统的患者，即一个人大多在医院，特别是在重症监护病房中感染埃夫菌，因此更有可能对许多抗生素产生耐药性。对各种抗生素产生耐药性和耐药菌株的出现是全球日益关注的问题。由于长期不加选择地使用抗生素，细菌产生了多重耐药性和广泛耐药性，这对医学界提出了新的挑战。为了治疗 Ef 引起的感染，测试了不同抗生素与纳米银 (AgNP) 的协同作用。在本研究中，AgNPs 的合成是从肺炎克雷伯菌的无细胞上清液中进行的。 AgNPs 使用多种技术进行表征，即紫外-可见分光光度法、透射电子显微镜和傅里叶变换红外光谱法。此外，还进行了工艺优化以提高 AgNP 的产量。此外，还测试了纳米颗粒的抗菌活性。此外，还评估了纳米颗粒与庆大霉素和氯霉素联合使用时的抗菌活性。结果表明，庆大霉素和氯霉素与AgNPs联用具有更好的抗菌效果。除此之外，还针对人红细胞 (RBC) 评估了 AgNP 的溶血活性。发现 AgNP 对红细胞无毒。 AgNps 与庆大霉素和氯霉素的集体作用比单独的 AgNps 更强，这表明这些组分具有协同作用。这些观察结果显示了 AgNP 与上述抗生素结合对抗 Ef 感染的潜力。粪肠球菌 (Ef) 是一种多重耐药细菌，对多种抗生素具有耐药性。由于耐药性不断增加，因此需要找到一种新方法来克服 Ef 引起的感染。纳米银 (AgNP) 与庆大霉素和氯霉素的联合作用对 Ef 的效果显着。此外，AgNP 对人类红细胞无毒，这证实了其无毒性质。使用的缩写：Ef：粪肠球菌、MDR：多药耐药性、AgNP：银纳米颗粒、Kp：肺炎克雷伯菌、RBC：红血球、ENP：工程纳米颗粒、FTIR：傅里叶变换红外光谱、TEM：透射电子显微镜、AgNO3：硝酸银、EDTA：乙二胺四乙酸、PBS：磷酸盐缓冲盐水。
Enterococcus faecalis (Ef) is a multidrug-resistant pathogenic bacteria associated with hospital-acquired infections. Ef is involved in a number of infectious diseases. It generally infects patients with the weekend immune system, i.e. a person mostly acquires Ef infections in the hospital, especially in intensive care units and thus, is more likely to be resistant to many antibiotics. Development of resistance against various antibiotics and emergence of drug-resistant strains is a growing global concern. Due to the unselective use of antibiotics for a long time multidrug resistant bacteria and extensively drug-resistant, which is now posing a new challenge to the medical community. To treat infections caused by Ef, the synergistic effect of different antibiotics with silver nanoparticles (AgNPs) was tested against Ef. In the present study, synthesis of AgNPs was carried out from the cell-free supernatant of Klebsiella pneumoniae. AgNPs were characterized using various techniques, namely, ultraviolet-visible spectrophotometry, transmission electron microscopy, and Fourier transform infrared spectroscopy. Moreover, process optimization was done for enhanced production of AgNPs. In addition, antimicrobial activity of the nanoparticles was also tested. Furthermore, the nanoparticles were evaluated for their antimicrobial activities in combination with gentamicin and chloramphenicol, against Ef. The results showed that the combination of gentamicin and chloramphenicol with AgNPs has a better antibacterial effect. To add to this, hemolytic activity of AgNPs was evaluated against human red blood corpuscles (RBCs). AgNPs were found to be nontoxic to RBCs. The collective effect of AgNps with Gentamicin and Chloramphenicol was more as compared to AgNps alone which indicate the synergistic effect of these components. These observations show the potential of AgNPs in combination with above-stated antibiotics against Ef infections. Enterococcus faecalis (Ef) is a multidrug-resistant bacteria with is resistant to wide range of antibiotics Due to this increasing resistance, there is a need to find a new approach to overcome the infections caused by Ef The combined effect of silver nanoparticles (AgNPs) with gentamicin and chloramphenicol was notably seen against Ef Furthermore, the AgNPs were nontoxic to the human red blood corpuscles which confirm its nontoxic nature. Abbreviations used: Ef: Enterococcus faecalis, MDR: Multidrug resistance, AgNPs: Silver nanoparticles, Kp: Klebsiella pneumoniae, RBCs: Red blood corpuscles, ENPs: Engineered nanoparticles, FTIR: Fourier transform infrared spectroscopy, TEM: Transmission electron microscopy, AgNO3: Silver nitrate, EDTA: Ethylenediaminetetraacetic acid, PBS: Phosphate-buffered saline.