Immunochemical quantitation of the third, fourth and fifth components of human complement: concentrations in the serum of healthy adults.
Immunochemical quantitation of the third, fourth and fifth components of human complement: concentrations in the serum of healthy adults.
复制标题
人类补体第三、第四和第五种成分的免疫化学定量：健康成人血清中的浓度。
DOI：
10.4049/jimmunol.99.6.1211
复制
发表时间：
1967
影响因子：
4.4
通讯作者：
H. Müller
中科院分区：
文献类型：
--
作者：
Peter F. Kohler;H. Müller
Immunochemical quantitation of the third, fourth and fifth components of human complement is feasible with the single radial immuno-diffusion technique. Serum component concentrations (mg/ml) in 25 healthy adults were: C′3, 1.23 ± 0.26 (0.86 to 1.84); C′4, 0.436 ± 0.118 (0.240 to 0.800) and C′5, 0.076 ± 0.024 (0.042 to 0.140). The experimental error of the quantitation was 10% for C′3 and C′4 and 15% for C′5.
The immunochemical quantitation is influenced by in vitro conditions which allow physicochemical alterations in complement proteins; inclusion of ethylenediaminetetraacetate in the agar and the use of fresh serum or serum stored at -70°C circumvents this problem.
