Expression pattern, genomic structure, and promoter analysis of the gene encoding stilbene synthase from Chinese wild Vitis pseudoreticulata.
Expression pattern, genomic structure, and promoter analysis of the gene encoding stilbene synthase from Chinese wild Vitis pseudoreticulata.
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DOI：
10.1093/jxb/erq447
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发表时间：
2011-05
影响因子：
6.9
通讯作者：
Weirong Xu;Yihe Yu;Q. Zhou;Jiahua Ding;Lingmin Dai;Xiaoqing Xie;Yan Xu;Chaohong Zhang;Yuejin Wan
中科院分区：
文献类型：
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作者：
Weirong Xu;Yihe Yu;Q. Zhou;Jiahua Ding;Lingmin Dai;Xiaoqing Xie;Yan Xu;Chaohong Zhang;Yuejin Wan
编码二苯乙烯合成酶(STS)的基因在许多生化和生理活动中起着核心作用，其代谢产物白藜芦醇对病原体具有广谱的抗性，并具有多种药理作用，尤其是抗癌作用。本文报道了中国野生抗白粉病葡萄品种的STS基因及其启动子功能的表达分析，并与两个对白粉病敏感的栽培葡萄品种葡萄进行了比较。Carignane和Thompson Seedless。我们发现STS在假网纹葡萄中有一种不寻常的表达模式，这与栽培种有明显的不同。序列比较表明，三种葡萄中编码STS的基因组DNA序列高度保守，但STS关键基序中存在一个新的残基突变，这一突变仅存在于假网纹葡萄中。此外，假网纹葡萄中的STS启动子与两个葡萄中发现的STS启动子具有显著不同的结构。3种启动子驱动的GUS在互隔交链孢菌、茉莉酸甲酯和伤害诱导转化烟草植株中的差异表达表明，假网纹病菌结构上不同的STS启动子参与了其特有的调控功能。我们还证明了来自其天然启动子的STS基因在转化烟草中的表达具有功能性，并保持了对病原菌的诱导能力。重要的是，假网纹葡萄基因组DNA-2在其天然启动子的诱导下表现出良好的诱导性和白藜芦醇的最高含量。这些发现加深了我们对STS在抗病葡萄中表达调控的理解，并为植物抗病遗传改良提供了一个新的病原菌诱导启动子系统。
The gene encoding stilbene synthase (STS) plays a central role in many biochemical and physiological actions, and its metabolite resveratrol possesses broad-spectrum resistance to pathogens, as well as diverse pharmacological properties, notably an anticancer effect. Here, we report the expression analysis of the gene encoding STS and its promoter function from a powdery mildew (PM)-resistant Chinese wild Vitis pseudoreticulata, and compare it with two PM-susceptible cultivated grapevines, Vitis vinifera cvs. Carignane and Thompson Seedless. We show an unusual expression pattern of STS in V. pseudoreticulata, which differs markedly from that of the cultivated species. Sequence comparisons reveal that the genomic DNA sequences encoding STS in the three grapevines are highly conserved, but a novel residue mutation within the key motif of STS is solely present in V. pseudoreticulata. Moreover, the STS promoter in V. pseudoreticulata displays a significantly different structure from that found in the two V. vinifera. The three promoter-driven GUS differential expression patterns in transformed tobacco plants induced with Alternaria alternata, methyl jasmonate, and wounding indicated that the structurally different STS promoter of V. pseudoreticulata is responsible for its specific regulatory function. We also demonstrate that the expression of STS genes from their native promoters are functional in transformed tobacco and retain pathogen inducibility. Importantly, the genomic DNA-2 of V. pseudoreticulata under its native promoter shows good induction and the maximum level of resveratrol content. These findings further our understanding of the regulation of STS expression in a resistant grapevine and provide a new pathogen-inducible promoter system for the genetic improvement of plant disease resistance.