Applications of microfluidics for molecular diagnostics.
Applications of microfluidics for molecular diagnostics.
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DOI：
10.1007/978-1-62703-134-9_20
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Gale BK
中科院分区：
文献类型：
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作者：
Jayamohan H;Sant HJ;Gale BK
在微流体装置中实施的诊断测定在过去十年中发展迅速，并且预计在未来几年内变得普遍。已经报道了数百种用于临床诊断和病原体检测的基于微流体的方法，其总体主题是快速和可定制的分析，这些分析具有潜在的成本效益。本章回顾了基于应用领域的分子诊断中的微流体技术，并简要回顾了微流体技术的一般情况。简要回顾了微加工的基本原理，并讨论了向聚合物制造器件的过渡。大多数当前的微流体诊断设备被设计为针对单一疾病，例如给定的癌症或各种病原体，并且这些集中的设备可能会有很大的市场;然而，分子诊断的未来在于高度多路复用的微流体设备，可以同时筛查潜在的数百种疾病。
Diagnostic assays implemented in microfluidic devices have developed rapidly over the past decade and are expected to become commonplace in the next few years. Hundreds of microfluidics-based approaches towards clinical diagnostics and pathogen detection have been reported with a general theme of rapid and customizable assays that are potentially cost-effective. This chapter reviews microfluidics in molecular diagnostics based on application areas with a concise review of microfluidics in general. Basic principles of microfabrication are briefly reviewed and the transition to polymer fabricated devices is discussed. Most current microfluidic diagnostic devices are designed to target a single disease, such as a given cancer or a variety of pathogens, and there will likely be a large market for these focused devices; however, the future of molecular diagnostics lies in highly multiplexed microfluidic devices that can screen for potentially hundreds of diseases simultaneously.