Swine generated by somatic cell nuclear transfer have increased incidence of intrauterine growth restriction (IUGR)
Swine generated by somatic cell nuclear transfer have increased incidence of intrauterine growth restriction (IUGR)
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DOI：
10.1089/clo.2006.0079
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发表时间：
2007-03-01
期刊：
影响因子：
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通讯作者：
Piedrahita, Jorge A.
中科院分区：
文献类型：
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作者：
Estrada, Jose;Sommer, Jeffrey;Piedrahita, Jorge A.
虽然体细胞核移植（SCNT）已在几个物种中取得成功，但许多妊娠失败，并在胎儿和围产期发现异常。在这项研究中，比较了SCNT和人工授精（AI）群体的窝仔数、平均出生体重、出生时存活的仔猪、死胎、木乃伊、第一周死亡的仔猪、宫内生长受限（IUGR）和大于胎龄（LGA）。将23只SCNT窝（143只个体）与112只AI窝（1300只个体）进行比较。AI和SCNT的平均窝仔数分别为11.5和6.2。AI组的窝重和经窝重调整的平均出生体重显著高于SCNT组（p < 0.05）。SCNT群体每窝IUGR数量显著增加（p < 0.01），AI和SCNT的LS均值分别为7.2 +/- 1.4和19.4 +/- 3.5，均值分别为8.0 +/- 10.8和15.5 +/- 24.5。此外，在SCNT人群中有较高的产后死亡率和死胎率的趋势。这些发现表明SCNT衍生的窝仔和AI窝仔之间存在一些差异。SCNT衍生的猪是研究IUGR所涉及的表观遗传因素和基因以及开发改善人类和动物胎儿生长的有效方法的极好模型。
While somatic cell nuclear transfer (SCNT) has been successful in several species, many pregnancies are lost and anomalies are found in fetal and perinatal stages. In this study SCNT and artificial inseminations (AI) populations were compared for litter size, average birth weight, piglets alive at birth, stillborn, mummies, dead at the first week, intrauterine growth restriction (IUGR) and large for gestational age (LGA). Twenty-three SCNT litters (143 individuals) were compared to 112 AI litters (1300 individuals). Litter size average was 11.5 for AI and 6.2 for SCNT. Litter weight and average birth weight adjusted by litter size were significantly (p < 0.05) higher in AI than in SCNT litters. The SCNT population had a significant (p < 0.01) increase in the number of IUGRs per litter with LSmeans 7.2 +/- 1.4 versus 19.4 +/- 3.5 and means 8.0 +/- 10.8 versus 15.5 +/- 24.5 for AI and SCNT, respectively. Additionally, there was a trend for higher postnatal mortality and stillbirths in the SCNT population. These findings demonstrate that there are some differences between SCNT-derived and AI litters. SCNT-derived pigs are excellent models to study epigenetic factors and genes involved in IUGRs, and to develop effective means to improve fetal growth in humans and animals.