Sequence and phylogenetic analysis of the complete mitochondrial genome of the flour beetle Tribolium castanaeum
Sequence and phylogenetic analysis of the complete mitochondrial genome of the flour beetle Tribolium castanaeum
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DOI：
10.1016/s1055-7903(02)00335-4
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发表时间：
2003-03-01
影响因子：
4.1
通讯作者：
Muqim, N
中科院分区：
文献类型：
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作者：
Friedrich, M;Muqim, N
我们描述了第一个完整的线粒体基因组序列的代表昆虫鞘翅目，面粉甲虫赤拟谷盗。赤拟谷盗线粒体基因组编码13种蛋白质、2种核糖体RNA和22种动物线粒体基因组典型的tRNA。它们的排列与黑腹果蝇（Drosophila melanogaster）中的排列相同，黑腹果蝇被认为是昆虫和甲壳类动物的祖先（Boore等人，1998; Hwang等人，2001年a）。核苷酸组成，氨基酸组成，和密码子的使用范围内的其他昆虫线粒体基因组中观察到的值。最显着的特点是使用TCT作为tRNA（Ser（AGN））（AGN）的反密码子，而不是GCT，这是在大多数其他节肢动物物种中使用的，以及在1431 bp长的控制区的特殊序列基序的相对稀缺。系统发育分析证实了解决能力的线粒体蛋白质组的保守区域的多样化事件，这之前出现的有翅昆虫，而很少的决议获得在基础perygote多样化的水平。进化较快的氨基酸位点的分区为鳞翅目与双翅目的结合提供了强有力的支持，这与单系的Mecopterida一致。(C)2002 Elsevier Science（美国）。All rights reserved.
We describe the first complete mitochondrial genome sequence from a representative of the insect order Coleoptera, the flour beetle Tribolium castaneum. The 15,881 bp long Tribolium mitochondrial genome encodes 13 putative proteins, two ribosomal RNAs and 22 tRNAs canonical for animal mitochondrial genomes. Their arrangement is identical to that in Drosophila melanogaster, which is considered ancestral for insects and crustaceans (Boore et at., 1998; Hwang et at., 2001 a). Nucleotide composition, amino acid composition, and codon usage fall within the range of values observed in other insect mitochondrial genomes. Most notable features are the use of TCT as tRNA(Ser(AGN))(AGN) anticodon instead of GCT, which is used in most other arthropod species, and the relative scarcity of special sequence motifs in the 1431 bp long control region. Phylogenetic analysis confirmed resolving power in the conserved regions of the mitochondrial proteome regarding diversification events, which predate the emergence of pterygote insects, while little resolution was obtained at the level of basal perygote diversification. The partition of faster evolving amino acid sites harbored strong support for joining Lepidoptera with Diptera, which is consistent with a monophyletic Mecopterida. (C) 2002 Elsevier Science (USA). All rights reserved.