Exclusively NOESY-based automated NMR assignment and structure determination of proteins
Exclusively NOESY-based automated NMR assignment and structure determination of proteins
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DOI：
10.1007/s10858-011-9502-8
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发表时间：
2011-06-01
影响因子：
2.7
通讯作者：
Guentert, Peter
中科院分区：
文献类型：
--
作者：
Ikeya, Teppei;Jee, Jun-Goo;Guentert, Peter
提出了一种全自动的方法，用于确定NMR溶液结构的蛋白质，专门使用NOESY光谱作为输入，避免了需要测量任何光谱，只获得共振分配，但没有结构信息。应用于两个小的蛋白质，该方法产生的结构与传统确定的结构非常吻合。
A fully automated method is presented for determining NMR solution structures of proteins using exclusively NOESY spectra as input, obviating the need to measure any spectra only for obtaining resonance assignments but devoid of structural information. Applied to two small proteins, the approach yielded structures that coincided closely with conventionally determined structures.