Regulation of PDGF and PDGF receptor in cultured dermal papilla cells and follicular keratinocytes of the human hair follicle
Regulation of PDGF and PDGF receptor in cultured dermal papilla cells and follicular keratinocytes of the human hair follicle
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DOI：
10.1034/j.1600-0625.2003.00089.x
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发表时间：
2003-10-01
影响因子：
3.6
通讯作者：
Blume-Peytavi, U
中科院分区：
文献类型：
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作者：
Kamp, H;Geilen, CC;Blume-Peytavi, U
血小板衍生生长因子（PDGF）是一种有效的促有丝分裂因子，对许多细胞类型，并已被证明是重要的卵泡发育和血管发生。在这项研究中，我们研究了PDGF因子及其相应的受体在间充质来源的毛乳头细胞（DPC）和上皮滤泡角质形成细胞（FK）的表达模式。两种类型的PDGF受体都在FK中表达，而DPC仅在蛋白质水平上表达PDGF受体β，这一发现也见于整个器官培养物。通过检测PDGF配体的表达，我们能够表明培养的FK合成PDGF-A和PDGF-B，而DPC仅表达PDGF-A。由于免疫调节细胞因子被证明影响毛发生长，我们研究了IL-1 β、IL-4、TNF-α、TGF-β和IFN-γ对培养的DPC和FK中PDGF因子表达水平的影响。有趣的是，我们可以通过诱导退行期的细胞因子如IL-1 β或IFN-γ显示出显著的下调作用，这表明PDGF信号传导可能参与了退行期的诱导。关于后一种假说的问题还有待于在全器官培养的进一步研究中阐明。
Platelet-derived growth factor ( PDGF) is a potent mitogenic factor for many cell types and has been shown to be important in follicular development and vasculogenesis. In this study, we examined the expression pattern of both PDGF factors and their corresponding receptors in mesenchyme-derived dermal papilla cells (DPCs) and epithelial follicular keratinocytes (FKs). Both types of PDGF receptors are expressed in FKs, whereas DPCs only express PDGF receptor beta on the protein level, a finding also seen in whole organ cultures. By examining the expression of PDGF ligands, we were able to show that cultured FKs synthesize both PDGF-A and PDGF-B, whereas, DPCs only express PDGF-A. As immunomodulatory cytokines were shown to affect hair growth, we investigated the effects of IL-1beta, IL-4, TNF-alpha, TGF-beta and IFN-gamma on the expression levels of PDGF factors in cultured DPCs and FKs. Interestingly, we could show a significant down-regulatory effect by catagen-inducing cytokines like IL-1beta or IFN-gamma, suggesting a possible involvement of PDGF signaling in the induction of catagen. The question concerning the latter hypothesis remains to be elucidated in further studies on whole organ cultures.