An insulin-like growth factor found in hepatopancreas implicates carbohydrate metabolism of the blue crab Callinectes sapidus
An insulin-like growth factor found in hepatopancreas implicates carbohydrate metabolism of the blue crab Callinectes sapidus
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DOI：
10.1016/j.ygcen.2014.01.012
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发表时间：
2014-04-01
影响因子：
2.7
通讯作者：
Chung, J. Sook
中科院分区：
文献类型：
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作者：
Chung, J. Sook
由甲壳动物高血糖激素（CHH）从窦腺释放到血淋巴中引起的高血糖是在经历应激条件的十足类甲壳动物中发生的标志性生理现象之一。然而，这种升高的葡萄糖水平恢复到静息水平的机制仍然未知。有趣的是，注意到的是蓝蟹，Callinectes sapidus的成年女性和成年男性之间的血淋巴葡萄糖的清除率的差异：前者具有更快的清除比后者。内源性胰岛素样分子的存在下，建议在C sapidus，因为牛胰岛素注射，显着降低血淋巴葡萄糖的水平，先前由浮现压力或葡萄糖注射升高。利用5'和3' RACE技术，从成年雌性智人的肝胰腺中分离到一个胰岛素样分子的全长cDNA，该cDNA与胰岛素样雄激素腺因子（IAG）的推定序列相同，但5'和3' UTR序列不同。使用CasIAG-hep的双链RNA（dsRNA-CasIAG-hep，10 μ g/注射）在10天时间内注射5次的敲低研究使CasIAG-hep表达降低了类似于50%。注射dsRNA-CasIAG-hep组的血淋巴葡萄糖水平也保持高于用dsRNA-绿色荧光蛋白（dsRNA-IAG-hep）或生理盐水处理的那些。最重要的是，注射dsRNA-CasIAG-hep的动物的肝胰腺含有的碳水化合物（葡萄糖、海藻糖和糖原）的量显著低于对照组，表明CasIAG-hep在甲壳类动物的碳水化合物代谢中的功能与脊椎动物的碳水化合物代谢相似。(C)2014爱思唯尔公司All rights reserved.
Hyperglycemia that is caused by the release of crustacean hyperglycemic hormone (CHH) from the sinus gland to hemolymph is one of the hallmark physiological phenomena, occurring in decapod crustaceans experiencing stressful conditions. However, the mechanism(s) by which such elevated glucose levels return to resting levels is still unknown. Interestingly, noted is a difference in the clearance rate of hemolymph glucose between adult females and adult males of the blue crab, Callinectes sapidus: the former with more rapid clearance than the latter. The presence of an endogenous-insulin-like molecule is suggested in C sapidus because an injection of bovine insulin, significantly reduces the levels of hemolymph glucose that were previously elevated by emersion stress or the glucose injection. Using 5' and 3' RACE, the full-length cDNA of an insulin-like molecule is isolated from the hepatopancreas of an adult female C sapidus and shows the same putative sequence of an insulin-like androgenic gland factor (IAG) but differs in 5' and 3' UTR sequences. A knock-down study using five injections of double-stranded RNA of CasIAG-hep (dsRNA-CasIAG-hep, 10 mu g/injection) over a 10-day period reduces CasIAG-hep expression by similar to 50%. The levels of hemolymph glucose are also kept higher in dsRNA-CasIAG-hep injected group than those treated with dsRNA-green fluorescent protein (dsRNA-IAG-hep) or saline. Most importantly, the hepatopancreas of dsRNA-CasIAG-hep injected animals contains amounts of carbohydrate (glucose, trehalose, and glycogen) significantly lower than those of control groups, indicating that the function of CasIAG-hep in carbohydrate metabolism in crustaceans is similar to carbohydrate metabolism in vertebrates. (C) 2014 Elsevier Inc. All rights reserved.