A class B scavenger receptor from Eriocheir sinensis (EsSR-B1) restricts bacteria proliferation by promoting phagocytosis
A class B scavenger receptor from Eriocheir sinensis (EsSR-B1) restricts bacteria proliferation by promoting phagocytosis
复制标题
中华绒螯蟹A类清道夫受体（EsSR-B1）通过促进吞噬作用限制细菌增殖
DOI：
10.1016/j.fsi.2017.09.034
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发表时间：
2017
影响因子：
4.7
通讯作者：
Li Wei-Wei
中科院分区：
文献类型：
--
作者：
Wu Yao-Meng;Yang Lei;Li Xue-Jie;Li Lu;Wang Qun;Li Wei-Wei
Scavenger receptors (SRs) are important pattern recognition receptors (PRRs), which play significant roles in host defense against pathogens by identifying pathogen-associated molecular patterns (PAMPs). In this study, we report the cloning and characterization of a SR fromEriocheir sinensis(EsSR-B1) which is a 500 amino acid protein encoded by a gene comprised of 2726 nucleotides with a 1503 bp open reading frame. The domains ofEsSR-B1 were found to be evolutionarily conserved.EsSR-B1 was widely detected in different tissues ofE. sinensisand significantly up-regulated in hemocytes after stimulation byStaphyloccocus aureusorVibrio parahaemolyticus. RecombinantEsSR-B1 protein could bind to bacteria and promote phagocytosis upon bacterial stimulation. Moreover, antimicrobial peptide expression was reduced inEsSR-B1-silenced hemocytes after challenge byS. aureusorV. parahaemolyticus. Thus,EsSR-B1 has a critical role in the binding of bacteria and subsequent promotion of hemocyte phagocytosis.
