Protective Effect of Zengye Decoction on Submandibular Glands in Nonobese Diabetic Mice
Protective Effect of Zengye Decoction on Submandibular Glands in Nonobese Diabetic Mice
复制标题
增液汤对非肥胖糖尿病小鼠颌下腺的保护作用。
DOI：
10.1007/s11655-014-1981-5
复制
发表时间：
2019-01-01
影响因子：
2.9
通讯作者：
Wang Yue
中科院分区：
文献类型：
--
作者：
Li Cheng-yin;Wu Su-ling;Wang Yue
目的探讨增液汤对非肥胖糖尿病(NOD)小鼠颌下腺的保护作用。方法27只NOD雌性小鼠随机分为3组：模型组、羟氯喹组和ZYD组。9只C57/B6小鼠作为正常组。习服1周后，HCQ组和ZYD组分别灌胃给予HCQ和ZYD，正常组和模型组给予生理盐水。观察唾液流率的变化。于20周龄处死4组小鼠。收集血清和SMG。采用双抗体夹心酶联免疫吸附试验检测血清细胞因子γ-干扰素、白介素10。苏木精-伊红染色观察颌下腺的组织学变化。结果与模型组比较，ZYD组大鼠唾液流量明显增加(P
ObjectiveTo investigate the protective effect of Zengye Decoction (???, ZYD) on the submandibular glands (SMGs) in nonobese diabetic (NOD) mice.MethodsTwenty-seven female NOD mice were randomly equally divided into 3 groups: the model group, the hydroxychloroquine (HCQ) group, and the ZYD group. Nine C57/B6 mice served as the normal group. After 1-week acclimation, the HCQ and ZYD groups were intragastrically administered with HCQ and ZYD, respectively, and the normal and model groups were administered with normal saline. Changes in the salivary flow rate were observed. Mice from all 4 groups were sacrificed at the age of 20 weeks. The serum and SMGs were collected. Serum cytokines gamma-interferon (IFN-), interleukin-10 (IL-10) were detected by enzyme-linked immunosorbent assay. Histological changes in the submandibular glands were examined by hematoxylin and eosin staining. The mRNA expression of IFN-, IL-10 and vasoactive intestinal peptide (VIP) in the submandibular glands were measured by real-time polymerase chain reaction.ResultsCompared with the model group, the salivary flow of the ZYD group significantly increased (P