Division of Labor Between Two Actin Nucleators-the Formin FH1 and the ARP2/3 Complex-in Arabidopsis Epidermal Cell Morphogenesis
Division of Labor Between Two Actin Nucleators-the Formin FH1 and the ARP2/3 Complex-in Arabidopsis Epidermal Cell Morphogenesis
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DOI：
10.3389/fpls.2020.00148
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发表时间：
2020-03-02
影响因子：
5.6
通讯作者：
Cvrckova, Fatima
中科院分区：
文献类型：
--
作者：
Cifrova, Petra;Oulehlova, Denisa;Cvrckova, Fatima
ARP 2/3复合物和formin是唯一已知的植物肌动蛋白成核剂。除了肌动蛋白相关的功能，这两个系统还调节微管组织和动力学。已知主要管家拟南芥I类膜靶向的ARPFH1（At3g25500）的缺失增加子叶铺面细胞成瓣，而影响ARP 2/3亚基的突变表现出相反的效果。在这里，我们研究的作用FH1和ARP2/3复合物亚基ARPC5（At4g01710）在表皮细胞形态发生的重点是路面细胞和毛状体使用的模型系统的单一FH1和arpc5，以及双FH1 arpc5突变体。虽然子叶路面细胞的形状在双突变体大多类似于单一arpc5突变体，分析真叶表皮形态，以及肌动蛋白和微管组织和动力学，揭示了更复杂的关系，两个系统和类似的，而不是拮抗，对一些参数的影响。与子叶相比，fh1和arpc5突变增加了第一真叶的铺面细胞和毛状体中肌动蛋白网络密度和细胞形状复杂性。因此，虽然这两个肌动蛋白成核系统在子叶铺面细胞的细胞形态发生的某些方面具有互补作用，但它们可能在其他细胞类型和发育阶段平行发挥作用。
The ARP2/3 complex and formins are the only known plant actin nucleators. Besides their actin-related functions, both systems also modulate microtubule organization and dynamics. Loss of the main housekeeping Arabidopsis thaliana Class I membrane-targeted formin FH1 (At3g25500) is known to increase cotyledon pavement cell lobing, while mutations affecting ARP2/3 subunits exhibit an opposite effect. Here we examine the role of FH1 and the ARP2/3 complex subunit ARPC5 (At4g01710) in epidermal cell morphogenesis with focus on pavement cells and trichomes using a model system of single fh1 and arpc5, as well as double fh1 arpc5 mutants. While cotyledon pavement cell shape in double mutants mostly resembled single arpc5 mutants, analysis of true leaf epidermal morphology, as well as actin and microtubule organization and dynamics, revealed a more complex relationship between the two systems and similar, rather than antagonistic, effects on some parameters. Both fh1 and arpc5 mutations increased actin network density and increased cell shape complexity in pavement cells and trichomes of first true leaves, in contrast to cotyledons. Thus, while the two actin nucleation systems have complementary roles in some aspects of cell morphogenesis in cotyledon pavement cells, they may act in parallel in other cell types and developmental stages.