MOLECULAR-CLONING AND SINGLE-CHANNEL PROPERTIES OF THE CYCLIC-NUCLEOTIDE GATED CHANNEL FROM CATFISH OLFACTORY NEURONS
MOLECULAR-CLONING AND SINGLE-CHANNEL PROPERTIES OF THE CYCLIC-NUCLEOTIDE GATED CHANNEL FROM CATFISH OLFACTORY NEURONS
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DOI：
10.1016/0896-6273(92)90107-o
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发表时间：
1992-01-01
期刊：
影响因子：
16.2
通讯作者：
CHESS, A
中科院分区：
文献类型：
--
作者：
GOULDING, EH;NGAI, J;CHESS, A
我们克隆了一种功能性 cDNA，编码在鲶鱼嗅觉感觉神经元中选择性表达的环核苷酸门控通道。环核苷酸门控通道与电压门控离子通道家族具有相同的序列和结构特征。这种同源性在跨膜区 S4（假定的电压传感器域）和 H5 域（被认为形成通道孔）中最为明显。我们描述了克隆鲶鱼通道的单通道特性，并将这些特性与本地鲶鱼嗅觉感觉神经元的通道进行了比较。该通道被 cAMP 和 cGMP 激活得同样好，仅表现出轻微的门控电压依赖性，并且表现出与电压门控 L 型钙通道观察到的类似的 pH 和电压依赖性亚电导状态。
We have cloned a functional cDNA encoding the cyclic nucleotide-gated channel selectively expressed in catfish olfactory sensory neurons. The cyclic nucleotide-gated channels share sequence and structural features with the family of voltage-gated ion channels. This homology is most evident in transmembrane region S4, the putative voltage sensor domain, and the H5 domain, thought to form the channel pore. We have characterized the single-channel properties of the cloned catfish channel and compared these properties with the channel in native catfish olfactory sensory neurons. The channel is activated equally well by cAMP and cGMP, shows only a slight voltage dependence of gating, and exhibits a pH- and voltage-dependent subconductance state similar to that observed for the voltage-gated L-type calcium channel.