Primary structure of the major pepsin inhibitor from the intestinal parasitic nematode Ascaris suum.
Primary structure of the major pepsin inhibitor from the intestinal parasitic nematode Ascaris suum.
复制标题
肠道寄生线虫猪蛔虫主要胃蛋白酶抑制剂的一级结构。
DOI：
10.1021/bi00484a003
复制
发表时间：
1990
期刊：
影响因子：
2.9
通讯作者：
Peanasky,RJ
中科院分区：
文献类型：
--
作者：
Martzen,MR;McMullen,BA;Smith,NE;Fujikawa,K;Peanasky,RJ
摘要：采用亲和层析和聚焦层析技术从猪蛔虫中分离出胃蛋白酶抑制剂。其氨基酸序列通过肽片段的自动Edman降解来确定。通过化学和酶促裂解吡啶乙基化蛋白质产生肽，并通过反相高效液相色谱法纯化。该抑制剂由149个残基组成，序列如下：QFLFSMSTGP 10 FICTVKDNQV 20 FVANLPWTML 30 EGDDIQVGKE 40-FAARVEDCTN 50 VKHDMAPTCT 60 KPPPFCGPQD 70 MKMFNFVGCS 80 VLGNKLFIDQ 90 KYVR-DLTAKD 100 HAEVQTFREK 110 IAAFEEQQENI 20 QPPSSGMPHG 130 AVPAGGLSPP 140 PPPSFCTVQ 149。分子量为16396。所有半胱氨酸均以二硫键接合：Cys（13）-Cys（59）、Cys（48）-Cys（66）和Cys（79）-Cys（146）。该蛋白可能由两个结构域组成，通过一个短的疏水区连接。这是第一个已测序的动物源性乙酰胆碱酯酶抑制剂。该序列与任何其他已知蛋白质都没有显著的同源性。世界上四分之一的人口和几乎所有的猪都感染了肠道内寄生线虫蛔虫
Revised Manuscript Received May 1, 1990 abstract: The major pepsin inhibitor from Ascaris suum was isolated by affinity chromatography and chromatofocusing. Its amino acid sequence was determined by automated Edman degradation of peptide fragments. Peptides were produced by chemical and enzymatic cleavage of pyridylethylated protein and were purified byreverse-phase high-performance liquid chromatography. The inhibitor consists of 149 residues with the following sequence: QFLFSMSTGP10FICTVKDNQV20FVANLPWTML30EGDDIQVGKE40-FAARVEDCTN50VKHDMAPTCT60KPPPFCGPQD70MKMFNFVGCS80VLGNKLFIDQ90KYVR-DLTAKD100HAEVQTFREK110IAAFEEQQENI20QPPSSGMPHG130AVPAGGLSPP140PPPSFCTVQ149. It has a molecular weight of 16 396. All cysteines are engaged as disulfide bonds: Cys (13)-Cys (59), Cys (48)-Cys (66), and Cys (79)-Cys (146). The protein is probably composed of two domains connected by a short hydrophobic region. This is the first aspartyl protease inhibitor of animal origin that has been sequenced. The sequence has no significant homology with any other known protein.^) ne-fourth of the world’s population and virtually all pigs are infected by the intestinal endoparasitic nematode Ascaris