Influence of the Headgroup on the Interaction of Poly(ethylene oxide)-Poly(propylene oxide) Block Copolymers with Lipid Bilayers.
Influence of the Headgroup on the Interaction of Poly(ethylene oxide)-Poly(propylene oxide) Block Copolymers with Lipid Bilayers.
复制标题
头基对聚环氧乙烷-聚环氧丙烷嵌段共聚物与脂质双层相互作用的影响。
DOI：
10.1021/acs.jpcb.0c00553
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Lodge,TimothyP
中科院分区：
文献类型：
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作者：
Zhang,Wenjia;Metzger,JosephM;Hackel,BenjaminJ;Bates,FrankS;Lodge,TimothyP
脂质头基在聚合物与脂质双层膜的缔合中起着重要作用。在此，我们报告了甘油头基与胆碱头基如何影响聚（环氧乙烷）-b-聚（环氧丙烷）（PEO-PPO）嵌段共聚物与脂质双层囊泡的相互作用。单层囊泡组成的磷脂酰胆碱和磷脂酰甘油在各种摩尔比被用作模型膜。的嵌段共聚物和脂质双层之间的相互作用进行了定量的脉冲场梯度核磁共振（PFG-NMR）的基础上的自由和绑定的聚合物的明显不同的迁移率。所有研究的聚合物物质均显示与1-棕榈酰-2-油酰-sn-甘油-3-磷酸-（1′-rac-甘油）钠盐（POPG）脂质体的结合显著高于与1-棕榈酰-2-油酰-sn-甘油-3-磷酸胆碱（POPC）脂质体的结合，表明与甘油头基的结合强于胆碱头基。直到混合的POPC/POPG脂质体的组合物含有超过20摩尔%的POPG，这种效果才变得显著。与POPG的增强的聚合物结合的一个合理的解释调用之间的氢键的作用的甘油头基和醚部分的聚合物。
The lipid headgroup plays an important role in the association of polymers with lipid bilayer membranes. Herein, we report how a glycerol headgroup versus a choline headgroup affects the interaction of poly(ethylene oxide)-b-poly(propylene oxide) (PEO-PPO) block copolymers with lipid bilayer vesicles. Unilamellar vesicles composed of phosphatidylcholine and phosphatidylglycerol at various molar ratios were used as model membranes. The interactions between the block copolymers and lipid bilayers were quantified by pulsed-field gradient nuclear magnetic resonance (PFG-NMR) based on the distinctly different mobilities of free and bound polymers. All the investigated polymer species showed significantly higher binding with 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1′-rac-glycerol) sodium salt (POPG) liposomes than with 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) liposomes, indicating stronger association with the glycerol headgroup compared to the choline headgroup. This effect did not become significant until the composition of mixed POPC/POPG liposomes contained more than 20 mol % POPG. A plausible explanation for the enhanced polymer binding with POPG invokes the role of hydrogen bonding between the glycerol headgroup and the ether moieties of the polymers.