Partially hydrolyzed guar gum alleviates small intestinal mucosal damage after massive small bowel resection along with changes in the intestinal microbiota
Partially hydrolyzed guar gum alleviates small intestinal mucosal damage after massive small bowel resection along with changes in the intestinal microbiota
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DOI：
10.1016/j.jpedsurg.2019.08.048
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发表时间：
2019-12-01
影响因子：
2.4
通讯作者：
Shimono, Ryuichi
中科院分区：
文献类型：
--
作者：
Fujii, Takayuki;Chiba, Yoichi;Shimono, Ryuichi
目的：短肠综合征与肠黏膜炎症和微生物生态失调相关，导致难治性并发症。部分水解瓜尔胶（PHGG）对肠道具有营养和抗炎作用。我们使用大鼠小肠切除（SBR）模型研究了PHGG是否改善小肠黏膜损伤并改变肠道微生物群。方法：将Sprague Dawley大鼠分为假手术组（sham）、sham /PHGG组、SBR组和SBR/PHGG组。第21天，对所有大鼠实施安乐死。为了评估小肠黏膜损伤，用形态计量学评估变性率，并用抗cd45抗体进行免疫组织化学检查。使用16S rRNA和短链脂肪酸生产分析粪便微生物群。结果：SBR组粘膜变性率明显高于Sham组和SBR/PHGG组。SBR组cd45阳性细胞数量明显高于Sham、Sham/PHGG或SBR/PHGG组。SBR/PHGG组毛螺科植物的相对丰度显著高于SBR组。结论：PHGG可减轻小肠黏膜损伤，其作用可能与调节肠道菌群有关。(C) 2019由爱思唯尔公司出版。
Purpose: Short bowel syndrome is associated with intestinal mucosal inflammation and microbial dysbiosis, leading to intractable complications. Partially hydrolyzed guar gum (PHGG) has trophic and anti-inflammatory effects on the intestine. We investigated whether PHGG ameliorates small intestinal mucosal damage and alters the intestinal microbiota using a rat small bowel resection (SBR) model.Methods: Sprague Dawley rats were divided into sham operation (Sham), Sham/PHGG, SBR, and SBR/PHGG groups. On day 21, all rats were euthanized. To assess small intestinal mucosal damage, the degeneration rate was morphometrically evaluated and immunohistochemically examined using anti-CD45 antibodies. Analyses of fecal microbiota using 16S rRNA and short-chain fatty acid production were also performed.Results: The mucosal degeneration rate was significantly higher in the SBR group than in the Sham or SBR/PHGG groups. The number of CD45-positive cells was significantly higher in the SBR group than in the Sham, Sham/PHGG, or SBR/PHGG groups. The relative abundance of family Lachnospiraceae was significantly higher in the SBR/PHGG group than in the SBR group.Conclusions: PHGG administration alleviated small intestinal mucosal damage which could be associated with modulation of the intestinal microbiota. (C) 2019 Published by Elsevier Inc.