Capping protein-controlled actin polymerization shapes lipid membranes.
Capping protein-controlled actin polymerization shapes lipid membranes.
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DOI：
10.1038/s41467-018-03918-1
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发表时间：
2018-04-24
影响因子：
16.6
通讯作者：
Bausch AR
中科院分区：
文献类型：
--
作者：
Dürre K;Keber FC;Bleicher P;Brauns F;Cyron CJ;Faix J;Bausch AR
Arp2/3 complex-mediated actin assembly at cell membranes drives the formation of protrusions or endocytic vesicles. To identify the mechanism by which different membrane deformations can be achieved, we reconstitute the basic membrane deformation modes of inward and outward bending in a confined geometry by encapsulating a minimal set of cytoskeletal proteins into giant unilamellar vesicles. Formation of membrane protrusions is favoured at low capping protein (CP) concentrations, whereas the formation of negatively bent domains is promoted at high CP concentrations. Addition of non-muscle myosin II results in full fission events in the vesicle system. The different deformation modes are rationalized by simulations of the underlying transient nature of the reaction kinetics. The relevance of the regulatory mechanism is supported by CP overexpression in mouse melanoma B16-F1 cells and therefore demonstrates the importance of the quantitative understanding of microscopic kinetic balances to address the diverse functionality of the cytoskeleton. Cell membrane protrusions and invaginations are both driven by actin assembly but the mechanism leading to different membrane shapes is unknown. Using a minimal system and modelling the authors reconstitute the deformation modes and identify capping protein as a regulator of both deformation types.
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影响因子：
16.6
作者：
Boujemaa-Paterski R;Suarez C;Klar T;Zhu J;Guérin C;Mogilner A;Théry M;Blanchoin L
通讯作者：
Blanchoin L
影响因子：
3.4
作者：
Carlsson, Anders E.;Bayly, Philip V.
通讯作者：
Bayly, Philip V.
DOI：
10.1073/pnas.1605350113
发表时间：
2016-10-25
影响因子：
11.1
作者：
Jung, Goeh;Alexander, Christopher J.;Hammer, John A.
通讯作者：
Hammer, John A.
DOI：
10.1083/jcb.146.6.1319
发表时间：
1999-09-20
期刊：
The Journal of cell biology
影响因子：
--
作者：
Egile C;Loisel TP;Laurent V;Li R;Pantaloni D;Sansonetti PJ;Carlier MF
通讯作者：
Carlier MF
影响因子：
3.3
作者：
Berro J;Pollard TD
通讯作者：
Pollard TD