Second generation sequencing of three STRs D3S1358, D12S391 and D21S11 in Danes and a new nomenclature for sequenced STR alleles
Second generation sequencing of three STRs D3S1358, D12S391 and D21S11 in Danes and a new nomenclature for sequenced STR alleles
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DOI：
10.1016/j.fsigen.2014.04.016
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发表时间：
2014-09-01
影响因子：
3.1
通讯作者：
Morling, Niels
中科院分区：
文献类型：
--
作者：
Gelardi, Chiara;Rockenbauer, Eszter;Morling, Niels
第二代测序 (SGS) 可能会彻底改变法医 STR 分型领域。实施基于 SGS 的法医调查方法的两个基本要求是 (1) 建立足够的频率数据库和 (2) 采用新的 STR 命名法。我们报告了 197 个不相关的丹麦人中三个 STR 位点的 STR 序列和等位基因频率：D3S1358、D12S391 和 D21S11。我们使用了新的 STR 命名法，该命名法描述了法医遗传学中使用的基因座名称、重复区域的长度除以重复长度（通常为 4 个核苷酸）以及扩增片段内可能的亚重复和 SNP 的详细序列信息。 (C) 2014 Elsevier Ireland Ltd. 保留所有权利。
Second generation sequencing (SGS) may revolutionize the field of forensic STR typing. Two of the essential requirements for implementation of an SGS based approach for forensic investigations are (1) establishment of adequate frequency databases and (2) adoption of a new STR nomenclature. We report the STR sequences and allele frequencies of three STR loci: D3S1358, D12S391 and D21S11 in 197 unrelated Danes. We used a new STR nomenclature that depicts the locus name used in forensic genetics, the length of the repeat region divided by the repeat length (typically 4 nucleotides) and detailed sequence information of possible sub-repeats and SNPs within the amplified fragment. (C) 2014 Elsevier Ireland Ltd. All rights reserved.