Ceramide and S1P Signaling in Embryonic Stem Cell Differentiation.
Ceramide and S1P Signaling in Embryonic Stem Cell Differentiation.
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DOI：
10.1007/7651_2017_43
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发表时间：
2018
期刊：
影响因子：
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通讯作者：
Bieberich E
中科院分区：
文献类型：
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作者：
Wang G;Spassieva SD;Bieberich E
具有生物活性的鞘脂是干细胞存活和分化的重要调节因子。最近，我们创造了术语“形态发生脂质”，用于在胚胎和出生后发育期间调节干细胞的鞘脂。鞘脂神经酰胺及其衍生物鞘氨醇-1-磷酸（S1 P）对胚胎干（ES）细胞分化具有协同和拮抗作用。我们在这里展示了简单的以及最先进的方法来分析鞘脂在分化ES细胞和讨论新的协议，使用神经酰胺和S1 P类似物的小鼠ES细胞向神经元和神经胶质细胞系的指导分化。
Bioactive sphingolipids are important regulators for stem cell survival and differentiation. Most recently, we have coined the term “morphogenetic lipids” for sphingolipids that regulate stem cells during embryonic and post-natal development. The sphingolipid ceramide and its derivative, sphingosine-1-phosphate (S1P), can act synergistically as well as antagonistically on embryonic stem (ES) cell differentiation. We show here simple as well as state-of-the art methods to analyze sphingolipids in differentiating ES cells and discuss new protocols to use ceramide and S1P analogs for the guided differentiation of mouse ES cells toward neuronal and glial lineage.