Emergence and transmission pathways of rapidly evolving evolutionary branch C4a strains of human enterovirus 71 in the Central Plain of China.
Emergence and transmission pathways of rapidly evolving evolutionary branch C4a strains of human enterovirus 71 in the Central Plain of China.
复制标题
中国中部平原的人类肠病毒71的快速进化分支C4a菌株的出现和传播途径。
DOI：
10.1371/journal.pone.0027895
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Xu W
中科院分区：
文献类型：
--
作者：
Zhang Y;Wang J;Guo W;Wang H;Zhu S;Wang D;Bai R;Li X;Yan D;Wang H;Zhang Y;Zhu Z;Tan X;An H;Xu A;Xu W
从2007年至今，中国中部平原（山东、安徽和河南省）多次发生手足口病（HFMD）大规模暴发。这些流行病的规模和严重程度每年都在增加，是中国大陆的一个主要公共卫生问题。根据HEV 71分离株的VP 1全序列进行系统发育分析，并构建贝叶斯马尔可夫链蒙特卡罗树。这些分析表明，中国中原地区的手足口病疫情是由至少5条HEV 71传播链引起的，并且该病毒在疫情之间的冬季继续传播和演变。1998年至2010年，中国大陆的HEV 71流行有2个阶段，2003-2004年从进化分支C4 b转移到C4 a。C4 a基因型的进化速率为4.99×10-3/位点/年，高于所有基因型的平均进化速率。最近的共同祖先估计中国集群日期为1994年10月和1993年11月的C4 a和C4 b进化分支，分别。与所有C4 a株相比，所有C4 b株基因组中的一个核苷酸替换（VP 1编码区nt 2503处A到C的回复，导致VP 1 -10的氨基酸替换：Gln到His）已经恢复。这些数据表明，C4 a HEV 71株传入中国中原地区负责最近的爆发。从组合序列和流行病学数据确定的HEV 71分离株之间的关系揭示了HEV 71循环的潜在季节动态。2007 - 2009年，中原地区至少有5个HEV 71毒株流行，其中山东和安徽毒株在冬季低传播季节经历了一个遗传瓶颈。
Large-scale outbreaks of hand, foot, and mouth disease (HFMD) occurred repeatedly in the Central Plain of China (Shandong, Anhui, and Henan provinces) from 2007 until now. These epidemics have increased in size and severity each year and are a major public health concern in mainland China. Phylogenetic analysis was performed and a Bayesian Markov chain Monte Carlo tree was constructed based on the complete VP1 sequences of HEV71 isolates. These analyses showed that the HFMD epidemic in the Central Plain of China was caused by at least 5 chains of HEV71 transmission and that the virus continued to circulate and evolve over the winter seasons between outbreaks. Between 1998 and 2010, there were 2 stages of HEV71 circulation in mainland China, with a shift from evolutionary branch C4b to C4a in 2003–2004. The evolution rate of C4a HEV71 was 4.99×10-3 substitutions per site per year, faster than the mean of all HEV71 genotypes. The most recent common ancestor estimates for the Chinese clusters dated to October 1994 and November 1993 for the C4a and C4b evolutionary branches, respectively. Compared with all C4a HEV71 strains, a nucleotide substitution in all C4b HEV71 genome (A to C reversion at nt2503 in the VP1 coding region, which caused amino acid substitution of VP1–10: Gln to His) had reverted. The data suggest that C4a HEV71 strains introduced into the Central Plain of China are responsible for the recent outbreaks. The relationships among HEV71 isolates determined from the combined sequence and epidemiological data reveal the underlying seasonal dynamics of HEV71 circulation. At least 5 HEV71 lineages circulated in the Central Plain of China from 2007 to 2009, and the Shandong and Anhui lineages were found to have passed through a genetic bottleneck during the low-transmission winter season.