Near-IR Laser and Raman Spectroscopy of Colon Mucosal Tissues: A Comparative Study on Metabolite Characterisations for Early Diagnosis of Inflammation and Ulceration
Near-IR Laser and Raman Spectroscopy of Colon Mucosal Tissues: A Comparative Study on Metabolite Characterisations for Early Diagnosis of Inflammation and Ulceration
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结肠粘膜组织的近红外激光和拉曼光谱：炎症和溃疡早期诊断代谢物特征的比较研究
DOI：
10.1109/icton.2018.8473594
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Addis J
中科院分区：
文献类型：
--
作者：
Addis J
粘膜组织在900 - 1300nm窗口内表现出扩展的近红外透明度，这与在800 - 1700nm范围内运行的光通信系统开发的技术有很好的重叠。在这项工作中，我们展示了一种激光，基于镱离子（Yb3+）掺杂的氧化碲玻璃增益介质，在1050 - 1055 nm的窄窗口中发射，首次用于肠组织检查的组织病理学显微应用。利用该激光器对肿瘤和健康人体肠道组织的光散射特性进行了表征。结果发现，这两种类型的组织在这个波长下都是高度透明的，肿瘤组织的激光散射明显低于健康组织。获得炎症（溃疡性结肠炎）和健康肠道组织的拉曼光谱。在类胡萝卜素和磷脂的浓度上发现了明显的差异，类胡萝卜素在炎症组是两倍强，而磷脂在炎症组则较低。肌红蛋白是另一种被发现的代谢物。通过多变量统计分析，我们发现健康组织和炎症组织之间的5个峰（拉曼位移1003、1155和1518 cm−1处的3个类胡萝卜素峰和1440和2762 cm−1处的2个磷脂峰）的强度存在显著差异。近红外散射和拉曼数据都可以区分健康和炎症或肿瘤组织，并显示了确定炎症存在的早期诊断工具的潜在方法。
Mucosal tissues exhibit extended near-IR transparency in the 900 – 1300 nm window, which overlaps nicely with the technologies developed for optical communication systems operating in the 800 – 1700 nm range. In this work, we have demonstrated a laser, based on an ytterbium-ion (Yb3+)-doped tellurium oxide glass gain medium, emitting in a narrow window of 1050 – 1055 nm, for use in the first instance as a histopathological microscopic application for bowel tissue examination. The light scattering properties of tumorous and healthy human bowel tissues were characterised using this laser. It was found that both types of tissue were highly transparent at this wavelength and that the laser scattering was significantly lower in the tumorous than that in the healthy tissue.Raman spectra were obtained for inflamed (ulcerative colitis) and healthy bowel tissues. A clear difference was identified in the concentrations of carotenoids, which were twice as strong in the inflamed, and in the phospholipids, which were lower in the inflamed. Myoglobin was another metabolite which was also identified. Using multivariate statistical analysis, we show that the intensities of five peaks (three carotenoid peaks at Raman shifts of 1003, 1155 and 1518 cm−1and two phospholipid peaks at 1440 and 2762 cm−1) are significantly different between healthy and inflamed tissues. Both the near-IR scattering and Raman data allow differentiation between the healthy and inflamed or tumorous tissues and show a potential methodology for early diagnosis tools for ascertaining the presence of inflammation.
影响因子：
0.9
作者：
Bashkatov, A. N.;Genina, E. A.;Tuchin, V. V.
通讯作者：
Tuchin, V. V.