Radioimmunoassay of Gastric Inhibitory Polypeptide
Radioimmunoassay of Gastric Inhibitory Polypeptide
复制标题
抑胃多肽的放射免疫分析
DOI：
10.1177/000456327801500138
复制
发表时间：
1978
影响因子：
2.2
通讯作者：
V. Marks
中科院分区：
文献类型：
--
作者：
L. Morgan;B. A. Morris;V. Marks
用抗猪胃抑制多肽(GIP)兔抗血清建立了测定人血浆中胃抑制多肽(GIP)的放射免疫分析方法。猪GIP也被用作标准，并用于生产125I标记的示踪剂。该方法能够区分血浆样品中110pg/mlGIP和0。与缩胆囊素、胰岛素、胰多肽、胰升糖素、分泌素和血管活性肠多肽的交叉反应可忽略不计。28例正常人隔夜空腹血浆GIP平均水平为203pg/ml(范围：无法检测到-420pg/ml)。在进食混合餐后的45分钟内，血浆GIP水平上升到1573 pg/ml的平均水平。
Summary A radioimmunoassay for the measurement of gastric inhibitory polypeptide (GIP) in unextracted plasma in man has been developed using a rabbit antiserum raised against porcine GIP. Porcine GIP was employed also as standard and to produce a 125I-labelled tracer. The assay was able to distinguish 110 pg/ml GIP from zero in plasma samples. Negligible cross-reactivity was demonstrated with cholecystokinin, insulin, pancreatic polypeptide, glucagon, secretin, and vasoactive intestinal polypeptide. The mean overnight fasting plasma GIP level in 28 normal subjects was 203 pg/ml (range: undetectable—420 pg/ml). Plasma GIP levels rose, within 45 minutes of eating a mixed meal, to a mean level of 1573 pg/ml.