NORADRENERGIC MODULATION OF NEURONAL RESPONSES TO N-METHYL-D-ASPARTATE IN THE VESTIBULAR NUCLEI: AN ELECTROPHYSIOLOGICAL AND IMMUNOHISTOCHEMICAL STUDY
NORADRENERGIC MODULATION OF NEURONAL RESPONSES TO N-METHYL-D-ASPARTATE IN THE VESTIBULAR NUCLEI: AN ELECTROPHYSIOLOGICAL AND IMMUNOHISTOCHEMICAL STUDY
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DOI：
10.1016/j.neuroscience.2014.01.054
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发表时间：
2014-04-18
期刊：
影响因子：
3.3
通讯作者：
Volsi, G. Li
中科院分区：
文献类型：
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作者：
Barresi, M.;Grasso, C.;Volsi, G. Li
在体内研究了n -甲基- d -天冬氨酸（NMDA）在去甲肾上腺素（NA）和/或其激动剂和拮抗剂微离子灌注大鼠前庭核（VN）中引起的兴奋反应。NMDA受体（NMDAR）在所有神经元中介导的na修饰兴奋反应的抛射；在59%的病例中，效果增强，其余41%的病例出现抑郁。除前庭外侧核外，所有VN均有增强，两种效果在相同数量的病例中均有记录。去甲肾上腺素能β受体激动剂异丙肾上腺素、β(1)特异性激动剂去多巴胺和α(2)激动剂可模拟NA对nmdar介导反应的增强作用。这些作用分别被β受体拮抗剂替马洛尔、β(1)拮抗剂阿替洛尔和α 2拮抗剂育亨宾阻断。相反，应用。受体激动剂唑啉和特异性α。，拮抗剂吡唑嗪分别模拟和部分拮抗NA诱导的nmdar介导的兴奋抑制。双标记免疫组织化学技术表明，在许多VN神经元中，NMDAR（特别是NR1和NR2亚基）与去甲肾上腺素能受体(α(1)、α（2)和β(1)）广泛共定位；在细胞核之间只发现了微小的差异。这些结果表明，NA可以对nmdar介导的VN兴奋性神经传递产生广泛的调节，从而改变细胞核内的突触可塑性。(c) 2014 ibro。Elsevier Ltd.出版。版权所有。
Excitatory responses evoked by N-methyl-D-aspartate (NMDA) in the vestibular nuclei (VN) of the rat were studied in vivo during microiontophoretic application of noradrenaline (NA) and/or its agonists and antagonists. Ejection of NA-modified excitatory responses mediated by NMDA receptors (NMDAR) in all neurons tested; the effect was enhancement in 59% of cases and depression in the remaining 41%. Enhancements prevailed in all VN with the exception of the lateral vestibular nucleus, where both effects were recorded in an equal number of cases. The enhancing actin of NA on NMDAR-mediated responses was mimicked by the noradrenergic beta-receptor agonist isoproterenol, the beta(1) specific agonist denopamine and the alpha(2) agonist clonidine. These effects were blocked respectively by the generic beta-receptor antagonist timolol, the beta(1) antagonist atenolol and the alpha2 antagonist yohimbine. In contrast, application of the alpha., receptor agonist cirazoline and the specific alpha., antagonist prazosin respectively mimicked and partially antagonized the depression of NMDAR-mediated excitations induced by NA. Double-labeling immunohistochemical techniques demonstrated broad colocalization of NMDAR (specifically NR1 and NR2 subunits) with noradrenergic receptors (alpha(1), alpha(2) and beta(1)) in many VN neurons; only minor differences were found between nuclei. These results indicate that NA can produce generalized modulation of NMDAR-mediated excitatory neurotransmission in VN, which may in turn modify synaptic plasticity within the nuclei. (C) 2014 IBRO. Published by Elsevier Ltd. All rights reserved.