Comparison of inflammatory urine markers in patients with interstitial cystitis and overactive bladder
Comparison of inflammatory urine markers in patients with interstitial cystitis and overactive bladder
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DOI：
10.1007/s00192-017-3547-5
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发表时间：
2018-07-01
影响因子：
1.8
通讯作者：
Yoshimura, Naoki
中科院分区：
文献类型：
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作者：
Furuta, Akira;Yamamoto, Tokunori;Yoshimura, Naoki
慢性炎症性疾病似乎是间质性膀胱炎（IC）和膀胱过度活动症（OAB）患者的共同特征。因此，我们测量了40个炎性尿液标记物在IC患者或没有Hunner病变（分别为HIC和NHIC）和OAB患者中，尿液收集自连续HIC患者、NHIC患者和年龄和性别匹配的OAB患者，这些患者没有IC、复发性尿路感染或膀胱癌病史。IC的诊断基于亚洲IC指南标准。使用MILLIPLEX免疫测定试剂盒测量尿液中代表性的40种炎性生长因子、细胞因子和趋化因子。通过非参数ANOVA随后多重比较检验确定各组之间这些标志物的统计学差异。血管内皮生长因子（VEGF）、白细胞介素-1 α（IL-1 α）、IL-6和趋化因子CCL 2、CCL 5、CXCL 1、CXCL 8和CXCL 10在HIC（n = 30）和NHIC（n = 30）患者中的表达均显著高于OAB（n = 28）患者。与NHIC患者相比，HIC患者CXCL 8和CXCL 10也明显升高。然而，各组间其他尿液标志物无显著差异。检测这些患者IC的VEGF、CXCL 10、CXCL 8、IL-1 α、CCL 5、CCL 2、IL-6和CXCL 1的曲线下面积分别为0.87、0.86、0.81、0.80、0.80、0.71、0.66、0.80、0.71、0.66和0.50。血管生成的增加-相关蛋白如VEGF和CXCL 10可能在IC的发生发展中具有重要的病理生理学意义。
Chronic inflammatory conditions seem to be a shared characteristic in patients with interstitial cystitis (IC) and overactive bladder (OAB). Thus, we measured 40 inflammatory urine markers in IC patients with or without Hunner's lesions (HIC and NHIC respectively) and OAB patients.Urine was collected from consecutive HIC patients, NHIC patients, and age and gender-matched OAB patients with no history of IC, recurrent urinary tract infection or bladder cancer. The diagnosis of IC was based on the Asian IC guideline criteria. A representative 40 inflammatory growth factors, cytokines, and chemokines in urine were measured using a MILLIPLEX immunoassay kit. Statistical differences in these markers among the groups were determined by nonparametric ANOVA followed by multiple comparison test. The diagnostic efficiency of these markers was measured using receiver operating characteristic analysis.Vascular endothelial growth factor (VEGF), interleukin-1 alpha (IL-1 alpha), IL-6, and chemokines including CCL2, CCL5, CXCL1, CXCL8, and CXCL10 were significantly increased in HIC (n = 30) and NHIC (n = 30) patients compared with OAB (n = 28) patients. The significant increases in CXCL8 and CXCL10 were also found in HIC patients compared with NHIC patients. However, there were no significant differences in the other urine markers among the groups. Area under the curves for VEGF, CXCL10, CXCL8, IL-1 alpha, CCL5, CCL2, IL-6, and CXCL1 to detect IC in these patients were 0.87, 0.86, 0.81, 0.80, 0.80, 0.71, 0.66, and 0.50 respectively.The increases in angiogenesis-associated proteins such as VEGF and CXCL10 may be pathophysiologically important for the development of IC.